| Project/Area Number |
26650112
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
Nonaka Shigenori 基礎生物学研究所, 時空間制御研究室, 准教授 (90435529)
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| Research Collaborator |
TANIGUCHI Atsushi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 繊毛 / 左右性 / 発生生物学 / 発生学 / 平面内細胞極性 / 基底小体 / 細胞生物学 / 発生・分化 / 細胞・組織 / 細胞極性 |
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| Outline of Final Research Achievements |
In mammalian development, leftward fluid flow generated by nodal cilia is crucial to future left-right asymmetry. The cilia beat in rotational pattern with different angular velocity between leftward and rightward phases, and the cause of the difference is not determined, manifestation of viscous resistance or active control based on the ciliary structure. That’s why we tried to find out circumferential orientation of the cilia, by determining basal foot and mother/daughter centrioles at the base of the cilia. Unfortunately, the reported marker of basal foot, ODF2, exhibited unexpected staining pattern and we couldn’t determine their exact orientation, while the orientation of the two centrioles (mother or daughter) seemed random, suggesting the viscous resistance model.
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