| Project/Area Number |
26650125
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Okayama University |
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Principal Investigator |
Miyaji Mary 岡山大学, 医歯(薬)学総合研究科, 助教 (50349255)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 核内構造 / 神経細胞分化 / 長大遺伝子 / 遺伝子間領域 / ゲノム環境 |
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| Outline of Final Research Achievements |
Vertebrate genomes contain a higher proportion of long genes compared to other organisms. Long genes are often located in AT-rich genomic environments and juxtaposed to long intergenic regions. Long genes are enriched in neuronal genes that are often mutated in psychic disorders like autism and schizophrenia. We hypothesized that the genomic environment of long genes are important for transcriptional regulation of long genes. In this study, we showed that topoisomerase IIbeta, in association with its partner protein (hnRNPU/SAF-A/SP120) is likely to be involved in the regulation of long gene expression. To test our model we utilized an immortalized neuronal cell line, RN33B, that is derived from brain stem raphe and transformed in vitro with a temperature-sensitive mutant of SV40 large T antigen. We obtained promising results indicating that this system is worthy of further analysis using genome-editing techniques.
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