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Analysis of a novel role of an RNA splicing factor in somatic hypermutation of the IgV gene

Research Project

Project/Area Number 26650126
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genetics/Chromosome dynamics
Research InstitutionOkayama University

Principal Investigator

Kanayama Naoki  岡山大学, 自然科学研究科, 准教授 (70304334)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords抗体 / 体細胞高頻度突然変異 / 親和性成熟 / スプライシング因子 / DT40 / SR / スプライシング / AID / SRタンパク質
Outline of Final Research Achievements

In this study, we analyzed molecular mechanisms for SRSF1-3 to contribute to somatic hypermutation of the IgV gene. It is revealed that although isoforms SRSF1 and SRSF1-3 are different only in the C-terminal region, the C-terminal region of SRSF1-3 is not essential for SHM, suggesting that the conserved region might be involved in SHM. In addition, this study indicated that SRSF1-3 may have a role in regulating the nuclear export of AID during SHM processes.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] Analysis of Distinct Roles of CaMKK Isoforms using STO-609-Resistant Mutants in Living Cells.2015

    • Author(s)
      Fujiwara Y, Hiraoka Y, Fujimoto T, Kanayama N, Magari M, Tokumitsu H.
    • Journal Title

      Biochemistry

      Volume: 54 Issue: 25 Pages: 3969-3977

    • DOI

      10.1021/acs.biochem.5b00149

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 過剰発現させたRNaseH1は核外へ隔離される2015

    • Author(s)
      154横山 和輝, 川口 祐加, 松山 雄磨, 河本 奈緒子, 成木 弘明, 徳光 浩, 曲 正樹, 金山 直樹
    • Organizer
      BMB2015
    • Place of Presentation
      神戸国際会議場・展示場
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニワトリSRSF1-3の抗体遺伝子変異における機能部位の探索2015

    • Author(s)
      155成木 弘明, 川口 祐加, 宮崎 誠士, 河本 奈緒子, 横山 和輝, 徳光 浩, 曲 正樹, 金山 直樹
    • Organizer
      BMB2015
    • Place of Presentation
      神戸国際会議場・展示場
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] SRSF1-3 promotes nuclear localization of AID by inhibiting nuclear export of AID
2015

    • Author(s)
      152KAWAGUCHI Yuka, KAWAMOTO Naoko, MIYAZAKI Satoshi, NARIKI Hiroaki, YOKOYAMA Kazuki, TOKUMITSU Hiroshi, MAGARI Masaki, KANAYAMA Naoki
    • Organizer
      2015日本免疫学会学術集会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] AID依存性の抗体高頻度変異の諸過程へのSRSF1-3 の寄与の解析2014

    • Author(s)
      河本 奈緒子, 川口 祐加, 金廣 優一, 鈴木 真理, 徳光 浩, 曲 正樹, 金山 直樹
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] AIDの核への局在化へのSRSF1-3の寄与2014

    • Author(s)
      川口 祐加, 宮崎 誠士, 河本 奈緒子, 徳光 浩, 曲 正樹, 金山 直樹
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 哺乳動物B細胞株におけるSRSF1-3およびBCL6のSHMへの関与2014

    • Author(s)
      松山 雄磨, 米田 万智, 鳥家 雄二, 川口 祐加, David Schatz, 徳光 浩, 曲 正樹, 金山 直樹
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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