Genomic and functional analysis for novel long-chain inverted repeats
| Project/Area Number |
26650127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAKAMI Hironori 九州大学, 大学院薬学研究院, 助教 (50403952)
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| Research Collaborator |
KASHO Kazutoshi 九州大学, 大学院薬学研究院, 助教 (90726019)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 遺伝学 / 遺伝子 / ゲノム / 細菌 / 生体生命情報学 |
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| Outline of Final Research Achievements |
Our analysis using genome sequence databases of Escherichia coli and the budding yeast indicated that long-chain inverted repeats are present at several sites. Mutants bearing deletions for these genomic sites were constructed and phenotypes of the resultant mutants were analyzed in detail. As a result, in Escherichia coli, a single site was suggested to have an important function in cell growth under certain conditions. This site was located in an intergenic region, and was indicated to be transcribed, yielding non-coding RNA. In addition, this non-coding RNA was suggested to be synthesized by transcriptional readthrough from a gene located upstream of the site.
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Report
(4 results)
Research Products
(7 results)
