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Genomic and functional analysis for novel long-chain inverted repeats

Research Project

Project/Area Number 26650127
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genetics/Chromosome dynamics
Research InstitutionKyushu University

Principal Investigator

Katayama Tsutomu  九州大学, 薬学研究院, 教授 (70264059)

Co-Investigator(Renkei-kenkyūsha) KAWAKAMI Hironori  九州大学, 大学院薬学研究院, 助教 (50403952)
Research Collaborator KASHO Kazutoshi  九州大学, 大学院薬学研究院, 助教 (90726019)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords遺伝学 / 遺伝子 / ゲノム / 細菌 / 生体生命情報学
Outline of Final Research Achievements

Our analysis using genome sequence databases of Escherichia coli and the budding yeast indicated that long-chain inverted repeats are present at several sites. Mutants bearing deletions for these genomic sites were constructed and phenotypes of the resultant mutants were analyzed in detail. As a result, in Escherichia coli, a single site was suggested to have an important function in cell growth under certain conditions. This site was located in an intergenic region, and was indicated to be transcribed, yielding non-coding RNA. In addition, this non-coding RNA was suggested to be synthesized by transcriptional readthrough from a gene located upstream of the site.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2014 Other

All Presentation (4 results) (of which Int'l Joint Research: 1 results) Remarks (3 results)

  • [Presentation] Genetic analyses of a unique, long intergenic region in E. coli2017

    • Author(s)
      呉 唯意, 土田 愛海, 川上 広宣, 加生 和寿, 片山 勉
    • Organizer
      KAIST-Kyushu University Joint Seminar
    • Place of Presentation
      休暇村志賀島(福岡県福岡市)
    • Year and Date
      2017-01-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞増殖に必須な長鎖逆方向反復配列の役割と保存性の推定2016

    • Author(s)
      川上 広宣, 土田 愛海, 竹原 通秋, 加生 和寿, 片山 勉
    • Organizer
      第13回21世紀大腸菌研究会
    • Place of Presentation
      グリーンピア南阿蘇(熊本県阿蘇郡)
    • Year and Date
      2016-06-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 大腸菌の増殖に必須な長鎖逆方向反復配列ELIXIR1の機能解析2014

    • Author(s)
      土田愛海, 川上 広宣, 加生 和寿, 末次 正幸, 片山 勉
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 大腸菌染色体の遺伝子間領域に存在する長鎖逆方向反復配列 ELIXIR の機能解析2014

    • Author(s)
      土田愛海, 川上 広宣, 末次 正幸, 片山 勉
    • Organizer
      第 11 回 21 世紀大腸菌研究会
    • Place of Presentation
      盛岡市
    • Year and Date
      2014-06-05 – 2014-06-06
    • Related Report
      2014 Research-status Report
  • [Remarks] 九州大学薬学研究院分子生物薬学分野

    • URL

      http://bunsei.phar.kyushu-u.ac.jp/

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
  • [Remarks] 九州大学研究者情報_片山 勉

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/K001101/index.html

    • Related Report
      2016 Annual Research Report 2015 Research-status Report 2014 Research-status Report
  • [Remarks] 九州大学薬学研究院分子生物薬学分野

    • URL

      http://bunsei.phar.kyushu-u.ac.jp

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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