Identification of circadian clock gene in eukaryotic cell

• Project/Area Number: 26650128
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Yokohama City University
• Principal Investigator: Kutsuna Shinsuke 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 准教授 (30315824)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物生理 / 遺伝子発現 / 概日時計 / シロイヌナズナ / 花弁 / 発生遺伝 / 概日リズム

Outline of Final Research Achievements

In this study, we analied flower movement under circadian clock. There are 3 of newly idenfified circadian flower movement mutants. These 3 mutants showed loss of flower closing and remarkable abnormality in circadian clock gene expression (CCA1, LHY1, and TOC1), suggesting the causative gene is a circadian clock gene. Genetic mapping experiment suggested the loacation of the mutation between 2 genetic molecular markers. There is no reported circadian clock gene in the genomic region. Whole genome sequencing analisis of the flower movement mutants determined several substitutions of base-pair among the suggested genetic marker region.

Research Products

• [Journal Article] Analysis of the fine-tuning of cyanobacterial circadian phase by monochromatic light and long day conditions. (2016)
  • Author(s): Kobayashi T, Obana Y, Kuboi N, Kitayama Y, Hayashi S, Oka M, Wada N, Arita K, Shimizu T, Sato M, Kanaly RA, Kutsuna S.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 1 Pages: 105-114
  • DOI: 10.1093/pcp/pcv177
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 花弁運動突然変異体における概日時計 遺伝子群の発現解析 (2016)
  • Author(s): 沓名伸介、瀬川祐貴1、小池杏奈、 小内 清、石浦正寛
  • Organizer: 日本時間生物学会
  • Place of Presentation: （愛知県・名古屋市）名古屋大学
  • Year and Date: 2016-11-11
• [Presentation] 花弁運動突然変異体における概日時計 遺伝子群の発現解析 (2016)
  • Author(s): 沓名伸介、瀬川祐貴1、小池杏奈、 小内 清、石浦正寛
  • Organizer: 日本遺伝学会
  • Place of Presentation: （静岡県・三島市）日本大学
  • Year and Date: 2016-09-24
• [Presentation] 海洋性シアノバクテリアの時計タンパク質発現解析 (2016)
  • Author(s): 森田朗嗣、沓名伸介 ほか
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] 植物の花弁運動突然変異体の概日時計遺伝子群の発現解析 (2016)
  • Author(s): 瀬川祐貴、沓名伸介 ほか
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] 海洋性シアノバクテリアの時計タンパク質発現解析 (2015)
  • Author(s): 森田朗嗣、沓名伸介 ほか
  • Organizer: 日本時間生物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-20
• [Presentation] 花弁運動突然変異体における概日時計遺伝子群の発現解析 (2015)
  • Author(s): 沓名伸介、瀬川祐貴、小池杏奈、小内 清、石浦正寛
  • Organizer: 日本遺伝学会
  • Place of Presentation: 東北大学
  • Year and Date: 2015-09-24
• [Presentation] Comparison of Circadian Clock Gene Expression in the Petal Movement (2015)
  • Author(s): 沓名伸介ほか
  • Organizer: 2.International ERATO Higashiyama Live-Holonics Symposium “Organogenesis from Eggs to Masure Plants”
  • Place of Presentation: 名古屋大学
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Molecular analysis of the circadian gene expression in the flower movement mutants in Arabidopsis thaliana (2015)
  • Author(s): 沓名伸介ほか
  • Organizer: 1.International Symposium of Correlative Gene System “Establishing Next-Generation Genetics”
  • Place of Presentation: 奈良 県立新公会堂
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] 真核生物の非転写・翻訳のフィードバック型概日時計因子の分子遺伝学的探索 (2014)
  • Author(s): 瀬川祐貴、ほか、沓名伸介
  • Organizer: 植物学会
  • Place of Presentation: 明治大学
  • Year and Date: 2014-09-12 – 2014-09-14