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Identification of circadian clock gene in eukaryotic cell

Research Project

Project/Area Number 26650128
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genetics/Chromosome dynamics
Research InstitutionYokohama City University

Principal Investigator

Kutsuna Shinsuke  横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 准教授 (30315824)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords植物生理 / 遺伝子発現 / 概日時計 / シロイヌナズナ / 花弁 / 発生遺伝 / 概日リズム
Outline of Final Research Achievements

In this study, we analied flower movement under circadian clock. There are 3 of newly idenfified circadian flower movement mutants. These 3 mutants showed loss of flower closing and remarkable abnormality in circadian clock gene expression (CCA1, LHY1, and TOC1), suggesting the causative gene is a circadian clock gene. Genetic mapping experiment suggested the loacation of the mutation between 2 genetic molecular markers. There is no reported circadian clock gene in the genomic region. Whole genome sequencing analisis of the flower movement mutants determined several substitutions of base-pair among the suggested genetic marker region.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Analysis of the fine-tuning of cyanobacterial circadian phase by monochromatic light and long day conditions.2016

    • Author(s)
      Kobayashi T, Obana Y, Kuboi N, Kitayama Y, Hayashi S, Oka M, Wada N, Arita K, Shimizu T, Sato M, Kanaly RA, Kutsuna S.
    • Journal Title

      Plant Cell Physiol.

      Volume: 57 Issue: 1 Pages: 105-114

    • DOI

      10.1093/pcp/pcv177

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 花弁運動突然変異体における概日時計 遺伝子群の発現解析2016

    • Author(s)
      沓名伸介、瀬川祐貴1、小池杏奈、 小内 清、石浦正寛
    • Organizer
      日本時間生物学会
    • Place of Presentation
      (愛知県・名古屋市)名古屋大学
    • Year and Date
      2016-11-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] 花弁運動突然変異体における概日時計 遺伝子群の発現解析2016

    • Author(s)
      沓名伸介、瀬川祐貴1、小池杏奈、 小内 清、石浦正寛
    • Organizer
      日本遺伝学会
    • Place of Presentation
      (静岡県・三島市)日本大学
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 海洋性シアノバクテリアの時計タンパク質発現解析2016

    • Author(s)
      森田朗嗣、沓名伸介 ほか
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 植物の花弁運動突然変異体の概日時計遺伝子群の発現解析2016

    • Author(s)
      瀬川祐貴、沓名伸介 ほか
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 海洋性シアノバクテリアの時計タンパク質発現解析2015

    • Author(s)
      森田朗嗣、沓名伸介 ほか
    • Organizer
      日本時間生物学会
    • Place of Presentation
      東京大学
    • Year and Date
      2015-11-20
    • Related Report
      2015 Research-status Report
  • [Presentation] 花弁運動突然変異体における概日時計遺伝子群の発現解析2015

    • Author(s)
      沓名伸介、瀬川祐貴、小池杏奈、小内 清、石浦正寛
    • Organizer
      日本遺伝学会
    • Place of Presentation
      東北大学
    • Year and Date
      2015-09-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Comparison of Circadian Clock Gene Expression in the Petal Movement2015

    • Author(s)
      沓名伸介ほか
    • Organizer
      2.International ERATO Higashiyama Live-Holonics Symposium “Organogenesis from Eggs to Masure Plants”
    • Place of Presentation
      名古屋大学
    • Year and Date
      2015-08-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular analysis of the circadian gene expression in the flower movement mutants in Arabidopsis thaliana2015

    • Author(s)
      沓名伸介ほか
    • Organizer
      1.International Symposium of Correlative Gene System “Establishing Next-Generation Genetics”
    • Place of Presentation
      奈良 県立新公会堂
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 真核生物の非転写・翻訳のフィードバック型概日時計因子の分子遺伝学的探索2014

    • Author(s)
      瀬川祐貴、ほか、沓名伸介
    • Organizer
      植物学会
    • Place of Presentation
      明治大学
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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