| Project/Area Number |
26650129
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Keio University |
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Principal Investigator |
Nose Toshiaki 慶應義塾大学, 医学部, 研究員 (70183902)
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| Research Collaborator |
中島 龍介 慶應義塾大学, 医学部, 博士課程4年
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 非コードRNA / 精子形成 / 減数分裂 / SINE配列 / 染色体 / non-coding RNA |
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| Outline of Final Research Achievements |
Transcripts derived from a small subset of SINEs have been known to be expressed during spermatogenesis, whereas their functions remain unknown. We have identified a non-coding RNA, R53, which starts to express shortly after germ cell induction from ES cells in vitro. The mature product of R53 is ca. 80 base-RNA containing a SINE/B1 motif. Surprisingly, R53 RNA shows an unique accumulation on the pairing chromosomes in meiotic cells at the MI/II-meiotic stage.
To know the function of R53 RNA, we examined knock-down effect on spermatogenesis by micro-injection of lenti-virus expressing R53 shRNA into testis tubules in an organ-culture. When testes of Acrosin-GFP tg mice were used as recipients and cultured for 2-3 weeks, differentiation of GFP-positive spermatids was significantly inhibited by R53 knock-down, suggesting that a specific SINE/B1-derived small RNA plays a critical role for regulating meiotic cell division and/or post-meiotic gene expression.
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