A Raman spectroscopic study of differentiating mechanism of PV membrane of symbiotic system with Paramecium bursaria and chlorella
| Project/Area Number |
26650133
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Yuuki 島根大学, 生物資源科学部, 准教授 (80582478)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMUKAI Makoto 島根大学, 生物資源科学部, 教授 (70186138)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ラマンスペクトル / 共生進化 / ミドリゾウリムシ / クロレラ / PV膜 / 食胞膜 / ラマン分光法 / 共生 / multivariate |
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| Outline of Final Research Achievements |
The PV menbrane of Paramecium bursaria is a key membrane to keep simbiosis sucessfully with co-living chlorella cells within it. In this study, we tried to measure Raman spectra of PV membrane, which is formed by differentiation from vacuole membrane of Paramecium bursaria. At first trial, we tried to measure Surface Enhanced Raman spectra of vacuole membrane and PV membrane using Ag nano-paricles. However, this trial was not sucessful because reliable and reproducible SERS spectra of these membrane were not obtained. Then we measured normal Raman spectra of vacuole membrae and succeeded in extract Raman spectral component attributable to vacuole membrane. This scucess means iti is teoretically possible to comapre Raman spectra of vacuole membrane and PV membrane.
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Report
(3 results)
Research Products
(49 results)
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[Journal Article] Comparison of gene expression of Paramecium bursaria with and without Chlorella variabilis symbionts2014
Author(s)
Kodama, Y., Suzuki, H., Dohra, H., Sugii, M., Kitazume, T., Yamaguchi, K., Shigenobu, S., and Fujishima, M.
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Journal Title
BMC Genomics
Volume: 15
Issue: 1
Pages: 183-183
DOI
NAID
Related Report
Peer Reviewed / Open Access
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