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DNA-incorporating microbial cells as agents of lateral gene transfer in extreme environments

Research Project

Project/Area Number 26650140
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionKyushu University

Principal Investigator

Yanagawa Katsunori  九州大学, 比較社会文化研究科(研究院), 研究員 (50599678)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords遺伝子水平伝播 / コンピテントセル / 熱水 / 進化 / 遺伝子
Outline of Final Research Achievements

An easy and rapid protocol to detect DNA-incorporating microbial cells by using flowcytometer was developed. This methodology was applied to microbial communities in several geothermal sites. Only one site showed the existence of naturally competent microbial cells, which uptake DNA molecules directly from extracellular environments into the cell bodies. In contrast, no uptake was observed after the long-term incubation of the samples. This study suggests that naturally competent cells are present in hot spring water and play an important role in horizontal gene transfer.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Int'l Joint Research] アリゾナ大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] スマトラ島北部の温泉に発達する多様な微生物マット2015

    • Author(s)
      杉原千耶,高島千鶴,柳川勝紀,森大器,奥村知世,Agung Harijoko
    • Journal Title

      地球科學

      Volume: 69 Pages: 141-142

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] スマトラ島北部の温泉に発達する多様な微生物マット2015

    • Author(s)
      杉原千耶,高島千鶴,柳川勝紀,森大器,奥村知世,Agung Harijoko
    • Journal Title

      地球科学

      Volume: 70

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Daily lamination formed by sulfur oxidizing bacteria and chloroflexus in a hot spring stromatolite2016

    • Author(s)
      Okumura, T, Takashima, C, Yanagawa, K, Sugihara, C, Harijoko, A and Kano, A
    • Organizer
      35th International Geological Congress
    • Place of Presentation
      Cape Town
    • Year and Date
      2016-08-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transition of microbial communities and laminated structures in travertines: a case study in northern Sumatra, Indonesia2016

    • Author(s)
      Sugihara, C., Kano, A., Yanagawa, K., Okumura, T., Takashima, C.
    • Organizer
      日本地球惑星科学連合2016年大会
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2016-05-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 北部スマトラ島の縞状トラバーチンと硫黄細菌2016

    • Author(s)
      杉原千耶,柳川勝紀,狩野彰宏,奥村知世,高島千鶴
    • Organizer
      地質学会西日本支部
    • Place of Presentation
      熊本大学
    • Year and Date
      2016-02-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 熱水噴出孔下に存在する生命圏の限界2015

    • Author(s)
      柳川勝紀,井尻暁,Anja Breuker,酒井早苗,三好陽子,川口慎介,野口拓郎,平井美穂,Axel Schippers,石橋純一郎,高木善弘,布浦拓郎,高井研
    • Organizer
      日本地球化学会第62回年会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] スマトラ島北部のトラバーチンに見られる微生物群集遷移2015

    • Author(s)
      杉原千耶,狩野彰宏,柳川勝紀,高島千鶴,奥村知世
    • Organizer
      日本古生物学会
    • Place of Presentation
      豊橋市自然史博物館
    • Year and Date
      2015-01-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 深海熱水噴出孔で生命の起源を探る2014

    • Author(s)
      柳川勝紀
    • Organizer
      第68回地団研九州総会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-08-22
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2017-05-10  

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