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Mechanism of siliceous shell construction in Paulinella chromatophora and the evolution of siliceous cell covering production

Research Project

Project/Area Number 26650142
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionUniversity of Tsukuba

Principal Investigator

ISHIDA Ken-ichiro  筑波大学, 生命環境系, 教授 (30282198)

Co-Investigator(Renkei-kenkyūsha) YOSHIDA Masaki  筑波大学, 生命環境系, 助教 (10449308)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords進化 / Paulinella / シリカバイオミネラリゼーション / 殻構築 / Paulinella chromatophora / 被殻形成 / 原生生物 / リザリア / 珪酸質被殻
Outline of Final Research Achievements

A transcriptome analysis and a proteome analysis on isolated siliceous scales were conducted in a siliceous testate amoeba Paulinella chromatophora (Rhizaria), in order to detect genes for siliceous shell construction and proteins associated with the siliceous scales. As a result, a silica transporter-like (sit-like) gene and several scale-associated proteins were detected. A phylogenyetic analysis on the sit genes suggested that the first organism that performed the silica-biomineralization was a testate amoeba like P. chromatophora.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Book (1 results)

  • [Journal Article] Fine-structure observation of the siliceous shell formation process in the testate amoeba Paulinella chromatophora2016

    • Author(s)
      Mami Nomura, Ken-ichiro Ishida
    • Journal Title

      Protist

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 有殻アメーバPaulinella chromatophoraにおける核分裂様式の解明2016

    • Author(s)
      野村真未・石田健一郎
    • Organizer
      日本藻類学会第40回大会
    • Place of Presentation
      日本歯科大学(東京都千代田区)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Paulinella chromatophora珪酸被殻構築過程における微小管・アクチン繊維の観察2014

    • Author(s)
      野村真未、石田健一郎
    • Organizer
      日本植物学会
    • Place of Presentation
      明治大学生田キャンパス、多摩区、川崎
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Fine Structure Observation during siliceous shell formation of a testate amoeba Paulinella chromatophora (Euglyphid)2014

    • Author(s)
      Mami Nomura and Ken-ichiro Ishida
    • Organizer
      ESF-EMBO共催 Biology of plastids : towards a blueprint for synthetic organelles
    • Place of Presentation
      プルトゥスク・ポーランド
    • Year and Date
      2014-06-21 – 2014-06-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 有殻アメーバ Paulinella chromatophoraにおける珪酸質被殻構築過程の微細構造および微小管・アクチン繊維の観察2014

    • Author(s)
      野村真未、石田健一郎
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      東大寺総合文化センター、奈良
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report
  • [Book] 進化の謎をゲノムで解く2015

    • Author(s)
      石田健一郎(監修:長谷部光泰)
    • Total Pages
      9
    • Publisher
      秀潤社
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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