Establishment of hornwort model system for land plant evo-devo studies

• Project/Area Number: 26650143
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Rikkyo University (2016-2017); Kanazawa University (2015); The University of Tokyo (2014)
• Principal Investigator: SAKAKIBARA Keiko 立教大学, 理学部, 准教授 (90590000)
• Research Collaborator: FRANGEDAKIS Eftychios 東京大学, 大学院理学系研究科・学術振興会, 特別研究員(PD)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 陸上植物 / 進化 / ゲノム / コケ植物 / 胞子体進化 / ツノゴケ / 形質転換系 / ゲノムシークエンス / 次世代シーケンサー

Outline of Final Research Achievements

Hornworts are one of bryophyte group, which locates at an important phylogenetic position in land plants. However the molecular genetic approaches or evo-devo research in hornworts were not well studied yet, because a hornwort model system was not available like other bryophyte groups, a moss Physcomitrella patens a liverwort and Marchantia polymorpha.
We tried to establish a hornwort model system using a hornwort species, Anthoceros agrestis, which can be induced all developmental stages of the life cycle within 3 months under experimental condition. As the results, we established stable transformation technique via Agrobacterium, generated Anthoceros agrestis draft genome assembly with paired-end library, and perform RNA sequencing using the gametophyte tissue. Also we found KNOX, LFY, and WOX genes, which were identified as sporophyte developmental genes in the moss Physcomitrella patens. We isolated promoter regions of these genes for further expression analysis.

Research Products

• [Int'l Joint Research] University of Cambridge(英国)
• [Journal Article] Evolution in the Cycles of Life (2016)
  • Author(s): Bowman J L, Sakakibara K, Furumizu C, Dierschke T
  • Journal Title: Annual Review of Genetics Volume: 50 Issue: 1 Pages: 133-154
  • DOI: 10.1146/annurev-genet-120215-035227
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 古い酒を新しい革袋に～preexisting gene regulatory network の転用による陸上植物のボディプラン革新 (2016)
  • Author(s): 石崎公庸 榊原恵子
  • Journal Title: BSJ-review Volume: 7B Pages: 47-54
  • Peer Reviewed / Open Access
• [Journal Article] TALE型ホメオボックス遺伝子族の進化による陸上植物複相の複雑化 (2016)
  • Author(s): 榊原恵子 古水千尋
  • Journal Title: BSJ-review Volume: 7B Pages: 66-77
  • Peer Reviewed / Open Access
• [Journal Article] Class III HD-Zip activity coordinates leaf development in Physcomitrella patens. (2016)
  • Author(s): Yip, H. K., Floyd, S. K., Sakakibara, K., Bowman, J. L.
  • Journal Title: Developmental Biology Volume: S0012-1606(15) Issue: 1 Pages: 30324-9
  • DOI: 10.1016/j.ydbio.2016.01.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Technological Innovations Give Rise to a New Era of Plant Evolutionary Developmental Biology (2016)
  • Author(s): Sakakibara, K.
  • Journal Title: Advances in Botanical Research Volume: 78 Pages: 3-35
  • DOI: 10.1016/bs.abr.2016.01.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem. (2015)
  • Author(s): Kinoshita, A, Hove, C.A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Bilou, I., Fukuda, H., Scheres, B., Heidstra, R., Sawa, S.
  • Journal Title: Development Volume: 142 Issue: 3 Pages: 444-453
  • DOI: 10.1242/dev.113167
  • Peer Reviewed / Open Access
• [Journal Article] Antagonistic Roles for KNOX1 and KNOX2 Genes in Patterning the Land Plant Body Plan Following an Ancient Gene Duplication (2015)
  • Author(s): Furumizu, C., Alvarez, J. P., Sakakibara, K., Bowman J. L.
  • Journal Title: PLOS Genetics Volume: 11 Issue: 2 Pages: e1004980-e1004980
  • DOI: 10.1371/journal.pgen.1004980
  • Peer Reviewed / Open Access
• [Journal Article] 陸上植物の複相の巨大化と複雑化に貢献したホメオボックス遺伝子の重複と機能分化 (2014)
  • Author(s): 榊原恵子
  • Journal Title: 細胞工学 Volume: 33 Pages: 872-877
  • NAID: 40020162174
• [Presentation] Making New Bryophyte Model System Using Genome Sequencing and Transformation Technique (2017)
  • Author(s): Keiko Sakakibara, Emiko Yoro, Tomomi Nakagawa, Eftychios Frangedakis, Masaki Shimamura and Tomoaki Nishiyama
  • Organizer: 65th NIBB conference
  • Int'l Joint Research
• [Presentation] ヒメツリガネゴケ胞子体幹細胞の制御機構 (2016)
  • Author(s): 榊原恵子
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-09-16
• [Presentation] KNOX遺伝子の遺伝子重複と新規機能獲得によってもたらされた陸上植物の世代交代の制御の分子機構 (2015)
  • Author(s): 榊原恵子、西山智明、塚谷裕一
  • Organizer: 日本進化学会第17回大会
  • Place of Presentation: 東京、中央大学
  • Year and Date: 2015-08-20
• [Presentation] KNOX gene family regulate the alternation of generations in land plants. (2015)
  • Author(s): Sakakibara K.
  • Organizer: Botany 2015
  • Place of Presentation: The Shaw Conference Centre, Edmonton, Alberta, Canada
  • Year and Date: 2015-07-25
  • Int'l Joint Research / Invited
• [Presentation] Evolution of the alternation of generations in Land Plants (2015)
  • Author(s): Sakakibara, K.
  • Organizer: Botany2015
  • Place of Presentation: The Shaw Conference Centre (Edmonton, Alberta, Canada)
  • Year and Date: 2015-07-25
  • Invited
• [Presentation] 世代交代を制御する転写因子KNOX2の下流遺伝子の探索 (2015)
  • Author(s): 榊原恵子、西山智明、塚谷裕一
  • Organizer: NGS現場の会第4回大会
  • Place of Presentation: 筑波、筑波国際会議場
  • Year and Date: 2015-07-01
• [Presentation] Identification of downstream genes of KNOX2 transcription factor that regulates alternations of generations in land plants (2015)
  • Author(s): 榊原恵子、西山智明、塚谷裕一
  • Organizer: 第56回日本植物生理学会
  • Place of Presentation: 東京農業大学 (東京都世田谷区)
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] KNOX遺伝子族の進化による陸上植物複相の複雑化 (2014)
  • Author(s): 榊原恵子、古水千尋、Bowman, J. L.
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学 (神奈川県川崎市)
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] コケ植物の再生能力とその制御因子 (2014)
  • Author(s): 榊原恵子
  • Organizer: 日本蘚苔類学会第43回大会
  • Place of Presentation: 奥入瀬渓流ホテル (青森県十和田市)
  • Year and Date: 2014-08-26 – 2014-08-28
• [Presentation] KNOX遺伝子は陸上植物の世代交代を制御する (2014)
  • Author(s): 榊原恵子
  • Organizer: 進化学会16回年会
  • Place of Presentation: 高槻現代劇場 (大阪府高槻市)
  • Year and Date: 2014-08-20 – 2014-08-24
• [Presentation] WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens (2014)
  • Author(s): Sakakibara, K., Reisewitz, P., Aoyama, T., Friedrich, T., Ando, S., Sato, Y., Nishiyama, T., Tamada, Y., Hiwatashi, Y., Kurata, T., Ishikawa, M., Deguchi, H., Rensing, S. A., Werr, W., Murata, T., Hasebe, M., and Laux, T.
  • Organizer: 日本発生生物学会第47回年会
  • Place of Presentation: Winc Aichi (Nagoya, Nichi)
  • Year and Date: 2014-05-27 – 2014-05-30
• [Book] 植物の世代交代制御因子の発見 (2016)
  • Author(s): 榊原恵子
  • Total Pages: 190
  • Publisher: 慶応義塾大学出版会