• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of hornwort model system for land plant evo-devo studies

Research Project

Project/Area Number 26650143
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionRikkyo University (2016-2017)
Kanazawa University (2015)
The University of Tokyo (2014)

Principal Investigator

SAKAKIBARA Keiko  立教大学, 理学部, 准教授 (90590000)

Research Collaborator FRANGEDAKIS Eftychios  東京大学, 大学院理学系研究科・学術振興会, 特別研究員(PD)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords陸上植物 / 進化 / ゲノム / コケ植物 / 胞子体進化 / ツノゴケ / 形質転換系 / ゲノムシークエンス / 次世代シーケンサー
Outline of Final Research Achievements

Hornworts are one of bryophyte group, which locates at an important phylogenetic position in land plants. However the molecular genetic approaches or evo-devo research in hornworts were not well studied yet, because a hornwort model system was not available like other bryophyte groups, a moss Physcomitrella patens a liverwort and Marchantia polymorpha.
We tried to establish a hornwort model system using a hornwort species, Anthoceros agrestis, which can be induced all developmental stages of the life cycle within 3 months under experimental condition. As the results, we established stable transformation technique via Agrobacterium, generated Anthoceros agrestis draft genome assembly with paired-end library, and perform RNA sequencing using the gametophyte tissue. Also we found KNOX, LFY, and WOX genes, which were identified as sporophyte developmental genes in the moss Physcomitrella patens. We isolated promoter regions of these genes for further expression analysis.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Int'l Joint Research] University of Cambridge(英国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Evolution in the Cycles of Life2016

    • Author(s)
      Bowman J L, Sakakibara K, Furumizu C, Dierschke T
    • Journal Title

      Annual Review of Genetics

      Volume: 50 Issue: 1 Pages: 133-154

    • DOI

      10.1146/annurev-genet-120215-035227

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 古い酒を新しい革袋に~preexisting gene regulatory network の転用による陸上植物のボディプラン革新2016

    • Author(s)
      石崎公庸 榊原恵子
    • Journal Title

      BSJ-review

      Volume: 7B Pages: 47-54

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] TALE型ホメオボックス遺伝子族の進化による陸上植物複相の複雑化2016

    • Author(s)
      榊原恵子 古水千尋
    • Journal Title

      BSJ-review

      Volume: 7B Pages: 66-77

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Class III HD-Zip activity coordinates leaf development in Physcomitrella patens.2016

    • Author(s)
      Yip, H. K., Floyd, S. K., Sakakibara, K., Bowman, J. L.
    • Journal Title

      Developmental Biology

      Volume: S0012-1606(15) Issue: 1 Pages: 30324-9

    • DOI

      10.1016/j.ydbio.2016.01.012

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Technological Innovations Give Rise to a New Era of Plant Evolutionary Developmental Biology2016

    • Author(s)
      Sakakibara, K.
    • Journal Title

      Advances in Botanical Research

      Volume: 78 Pages: 3-35

    • DOI

      10.1016/bs.abr.2016.01.001

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem.2015

    • Author(s)
      Kinoshita, A, Hove, C.A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Bilou, I., Fukuda, H., Scheres, B., Heidstra, R., Sawa, S.
    • Journal Title

      Development

      Volume: 142 Issue: 3 Pages: 444-453

    • DOI

      10.1242/dev.113167

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Antagonistic Roles for KNOX1 and KNOX2 Genes in Patterning the Land Plant Body Plan Following an Ancient Gene Duplication2015

    • Author(s)
      Furumizu, C., Alvarez, J. P., Sakakibara, K., Bowman J. L.
    • Journal Title

      PLOS Genetics

      Volume: 11 Issue: 2 Pages: e1004980-e1004980

    • DOI

      10.1371/journal.pgen.1004980

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 陸上植物の複相の巨大化と複雑化に貢献したホメオボックス遺伝子の重複と機能分化2014

    • Author(s)
      榊原恵子
    • Journal Title

      細胞工学

      Volume: 33 Pages: 872-877

    • NAID

      40020162174

    • Related Report
      2014 Research-status Report
  • [Presentation] Making New Bryophyte Model System Using Genome Sequencing and Transformation Technique2017

    • Author(s)
      Keiko Sakakibara, Emiko Yoro, Tomomi Nakagawa, Eftychios Frangedakis, Masaki Shimamura and Tomoaki Nishiyama
    • Organizer
      65th NIBB conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒメツリガネゴケ胞子体幹細胞の制御機構2016

    • Author(s)
      榊原恵子
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] KNOX遺伝子の遺伝子重複と新規機能獲得によってもたらされた陸上植物の世代交代の制御の分子機構2015

    • Author(s)
      榊原恵子、西山智明、塚谷裕一
    • Organizer
      日本進化学会第17回大会
    • Place of Presentation
      東京、中央大学
    • Year and Date
      2015-08-20
    • Related Report
      2015 Research-status Report
  • [Presentation] KNOX gene family regulate the alternation of generations in land plants.2015

    • Author(s)
      Sakakibara K.
    • Organizer
      Botany 2015
    • Place of Presentation
      The Shaw Conference Centre, Edmonton, Alberta, Canada
    • Year and Date
      2015-07-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Evolution of the alternation of generations in Land Plants2015

    • Author(s)
      Sakakibara, K.
    • Organizer
      Botany2015
    • Place of Presentation
      The Shaw Conference Centre (Edmonton, Alberta, Canada)
    • Year and Date
      2015-07-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 世代交代を制御する転写因子KNOX2の下流遺伝子の探索2015

    • Author(s)
      榊原恵子、西山智明、塚谷裕一
    • Organizer
      NGS現場の会第4回大会
    • Place of Presentation
      筑波、筑波国際会議場
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Identification of downstream genes of KNOX2 transcription factor that regulates alternations of generations in land plants2015

    • Author(s)
      榊原恵子、西山智明、塚谷裕一
    • Organizer
      第56回日本植物生理学会
    • Place of Presentation
      東京農業大学 (東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] KNOX遺伝子族の進化による陸上植物複相の複雑化2014

    • Author(s)
      榊原恵子、古水千尋、Bowman, J. L.
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学 (神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] コケ植物の再生能力とその制御因子2014

    • Author(s)
      榊原恵子
    • Organizer
      日本蘚苔類学会第43回大会
    • Place of Presentation
      奥入瀬渓流ホテル (青森県十和田市)
    • Year and Date
      2014-08-26 – 2014-08-28
    • Related Report
      2014 Research-status Report
  • [Presentation] KNOX遺伝子は陸上植物の世代交代を制御する2014

    • Author(s)
      榊原恵子
    • Organizer
      進化学会16回年会
    • Place of Presentation
      高槻現代劇場 (大阪府高槻市)
    • Year and Date
      2014-08-20 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens2014

    • Author(s)
      Sakakibara, K., Reisewitz, P., Aoyama, T., Friedrich, T., Ando, S., Sato, Y., Nishiyama, T., Tamada, Y., Hiwatashi, Y., Kurata, T., Ishikawa, M., Deguchi, H., Rensing, S. A., Werr, W., Murata, T., Hasebe, M., and Laux, T.
    • Organizer
      日本発生生物学会第47回年会
    • Place of Presentation
      Winc Aichi (Nagoya, Nichi)
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Research-status Report
  • [Book] 植物の世代交代制御因子の発見2016

    • Author(s)
      榊原恵子
    • Total Pages
      190
    • Publisher
      慶応義塾大学出版会
    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-06-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi