High-throughput DNA barcoding for foodweb analysis
| Project/Area Number |
26650144
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Kyoto University |
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Principal Investigator |
Toju Hirokazu 京都大学, 人間・環境学研究科(研究院), 助教 (60585024)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 生態学 / 群集 / 食物網 / ネットワーク / DNAバーコーディング / クモ / 群集生態学 |
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| Outline of Final Research Achievements |
In this research project, I developed a pipeline for detecting prey DNA in predator samples through high-throughput DNA barcoding based on next-generation sequencing. The pipeline consists of improved methods of sampling, DNA extraction, PCR, next-generation sequencing, bioinformatics, and community ecological statistical analyses. Because the pipeline is based on the universal DNA barcoding system (http://www.claident.org/), it is applicable to various types of interspecific interaction systems (e.g., food webs) involving various taxonomic groups of organisms.
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Report
(3 results)
Research Products
(4 results)
