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Strategy for obtaining pure culture of candidate division microorganisms in the environments

Research Project

Project/Area Number 26650145
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionHiroshima University

Principal Investigator

Kindaichi Tomonori  広島大学, 工学(系)研究科(研究院), 助教 (10379901)

Co-Investigator(Kenkyū-buntansha) AOI YOSHITERU  広島大学, サステナブル・ディベロップメント実践研究センター, 特任講師 (40386636)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords群集・生態系多様性 / 微生物ダークマター / 分離培養
Outline of Final Research Achievements

In this study, candidate phyla (TM7, OD1, SR1, and BD1-5), which were frequently detected using next generation sequencing for activated sludge samples. The phylogenetic tree at the phylum level could be constructed and FISH probes were newly designed and optimized. MAR-FISH analysis revealed the organic compound that TM7 bacteria can be utilized for their growth.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Int'l Joint Research] Aalborg University(Denmark)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Phylogenetic diversity and ecophysiology of candidate phylum Saccharibacteria in activated sludge2016

    • Author(s)
      Tomonori Kindaichi, Shiro Yamaoka, Ryohei Uehara, Noriatsu Ozaki, Akiyoshi Ohashi, Mads Albertsen, Per H. Nielsen, Jeppe L. Nielsen
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 92 Issue: 6 Pages: 1-11

    • DOI

      10.1093/femsec/fiw078

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 活性汚泥中に存在する糸状性細菌Candidatus Saccharibacteriaの集積培養2015

    • Author(s)
      上原 亮平, 金田一 智規, 尾崎 則篤, 大橋 晶良
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Candidatus Saccharibacteria の生理学的特性の解析と分離培養の試み2014

    • Author(s)
      上原 亮平, 金田一 智規, 尾崎 則篤, 大橋 晶良
    • Organizer
      環境微生物系学会合同大会
    • Place of Presentation
      浜松市
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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