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Mitochondrial genome complete sequencing and technological development of computer scientific "cell fractionation method" in fern

Research Project

Project/Area Number 26650146
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionJapan Women's University

Principal Investigator

Ootsuki Ryo  日本女子大学, 理学部, 研究員 (10646962)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords次世代シーケンス / シダ植物 / ミトコンドリアゲノム / 無配生殖 / 次世代シーケンサー / ゲノム / ミトコンドリア / ヤブソテツ
Outline of Final Research Achievements

In this study, we succeeded in determining the full length sequence of chloroplast of Cyrtomium fortunei by Next Generation Sequencing data (NGS). In mitochondrial genomes, we were able to determine the full length sequence of the major genes. But, the complete mitochondrial genome sequence which containing all the intergenic regions as originally planned could not be determined. Because, there may be multiple structural polymorphism (multipartite structure) in the mitochondrial genome.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] "PAFFT: A new homology search algorithm for third-generation sequencers."2015

    • Author(s)
      Misawa, Kazuharu, and Ryo Ootsuki.
    • Journal Title

      Genomics

      Volume: 106.5 Pages: 265-267

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] イチョウ(Ginkgo biloba)に見られる2種類の葉緑体ゲノム2017

    • Author(s)
      大槻涼 関本弘之
    • Organizer
      日本植物分類学会 第16回大会
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2017-03-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヤブソテツ(Cyrtomium fortunei)オルガネラゲノムにおけるマルチパータイト構造の解析2016

    • Author(s)
      大槻涼 関本弘之
    • Organizer
      日本植物学会 第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 4^20 1兆通りの中の“使われていない”配列2016

    • Author(s)
      大槻涼 関本弘之
    • Organizer
      日本進化学会 第18回大会
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヤブソテツにおける葉緑体全長配列の解読2015

    • Author(s)
      大槻涼 関本弘之 三澤計治
    • Organizer
      日本植物分類学会第14回大会
    • Place of Presentation
      福島大学(福島県福島市)
    • Year and Date
      2015-03-05 – 2015-03-08
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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