Mitochondrial genome complete sequencing and technological development of computer scientific "cell fractionation method" in fern
Project/Area Number |
26650146
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biodiversity/Systematics
|
Research Institution | Japan Women's University |
Principal Investigator |
Ootsuki Ryo 日本女子大学, 理学部, 研究員 (10646962)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 次世代シーケンス / シダ植物 / ミトコンドリアゲノム / 無配生殖 / 次世代シーケンサー / ゲノム / ミトコンドリア / ヤブソテツ |
Outline of Final Research Achievements |
In this study, we succeeded in determining the full length sequence of chloroplast of Cyrtomium fortunei by Next Generation Sequencing data (NGS). In mitochondrial genomes, we were able to determine the full length sequence of the major genes. But, the complete mitochondrial genome sequence which containing all the intergenic regions as originally planned could not be determined. Because, there may be multiple structural polymorphism (multipartite structure) in the mitochondrial genome.
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Report
(4 results)
Research Products
(5 results)