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Metagenomic analysis of phages in the termite gut and potential for application in pest control

Research Project

Project/Area Number 26650158
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionTokyo Institute of Technology

Principal Investigator

Hongoh Yuichi  東京工業大学, 生命理工学院, 教授 (90392117)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords共生 / 腸内細菌 / 昆虫 / シロアリ / ウイルス / ファージ / ゲノム / メタゲノム / CRISPR / 害虫 / ゲノミクス / 群集構造 / 難培養微生物
Outline of Final Research Achievements

Termites feed exclusively on dead plant matter. They play important roles in the global carbon cycle, although they are also destructive pests of wooden buildings. Their ability to digest lignocellulose is mostly attributable to the symbiotic gut microorganisms. In this study, a bacteriophage community that infects the symbiotic gut bacteria was comprehensively analyzed, and specific bacterial hosts of some phages were successfully identified.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (27 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (20 results) (of which Int'l Joint Research: 1 results,  Invited: 7 results) Remarks (1 results)

  • [Int'l Joint Research] University of Sydney(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Discovery and Complete Genome Sequence of a Bacteriophage from an Obligate Intracellular Symbiont of a Cellulolytic Protist in the Termite Gut2017

    • Author(s)
      Pramono A.K., Kuwahara H., Itoh T., Toyoda A., Yamada A., Hongoh Y
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 2 Pages: 112-117

    • DOI

      10.1264/jsme2.ME16175

    • NAID

      130006789871

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of intracellular “Ca. Endomicrobium trichonymphae” genomovars illuminates the requirement and decay of defense systems against foreign DNA2016

    • Author(s)
      Izawa, K., H. Kuwahara, K. Kihara, M. Yuki, N. Lo, T. Ito, M. Ohkuma, Y. Hongoh
    • Journal Title

      Genome Biol. Evol.

      Volume: 8(10) Issue: 10 Pages: 3099-3107

    • DOI

      10.1093/gbe/evw227

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Dominant ectosymbiotic bacteria of cellulolytic protists in the termite gut also have the potential to digest lignocellulose2015

    • Author(s)
      Yuki M., Kuwahara H., Shintani M., Izawa K., Sato T., Starns D., Hongoh Y., Ohkuma M.
    • Journal Title

      Environmental Microbiology

      Volume: 17 Issue: 12 Pages: 4942-4953

    • DOI

      10.1111/1462-2920.12945

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Dysgonomonas termitidis sp. nov., isolated from the gut of the subterranean termite Reticulitermes speratus2015

    • Author(s)
      Pramono A.K., Sakamoto M., Iino T., Hongoh Y., Ohkuma M.
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 65 Issue: Pt_2 Pages: 681-685

    • DOI

      10.1099/ijs.0.070391-0

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distribution and evolution of nitrogen fixation genes in the phylum Bacteroidetes.2015

    • Author(s)
      Inoue J., Oshima K., Suda W., Sakamoto M., Iino T., Noda S., Hongoh Y., Hattori M., Ohkuma M.
    • Journal Title

      Microbes and Environments

      Volume: 30 Pages: 44-50

    • NAID

      130005061711

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 1細胞ゲノムとメタゲノムから読み解くシロアリ腸内多重共生系2017

    • Author(s)
      本郷裕一
    • Organizer
      平成28年度EIIRISプロジェクト研究成果報告会
    • Place of Presentation
      豊橋技大、豊橋
    • Year and Date
      2017-03-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] シロアリ腸内ファージメタゲノム解析と宿主腸内細菌の同定2016

    • Author(s)
      麥島雄太、伊澤和輝、河合幹彦、桑原宏和、雪真弘、大熊盛也、本郷裕一
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロアリ腸内多重共生系の進化2016

    • Author(s)
      本郷裕一
    • Organizer
      日本進化学会第18回大会
    • Place of Presentation
      東工大、東京
    • Year and Date
      2016-08-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 対ファージ防衛機構が示すEndomicrobium属細胞内共生細菌の適応進化2016

    • Author(s)
      伊澤和輝、麥島雄太、桑原宏和、河合幹彦、木原久美子、Nathan Lo、雪真弘、伊藤武彦、大熊盛也、本郷裕一
    • Organizer
      第18回日本進化学会大会
    • Place of Presentation
      東工大、東京
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロアリと絶対共生する腸内微生物群集の進化と機能2016

    • Author(s)
      本郷裕一
    • Organizer
      第27回日本生体防御学会総会
    • Place of Presentation
      九大、福岡
    • Year and Date
      2016-07-08
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Single cell genomics deciphers the complex symbiotic system in the termite gut microbiota2016

    • Author(s)
      Yuichi Hongoh
    • Organizer
      第11回国際ゲノム会議
    • Place of Presentation
      一橋講堂
    • Year and Date
      2016-05-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ゲノムから読み解くシロアリ腸内難培養微生物群集の多 重共生機構2016

    • Author(s)
      本郷裕一
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] シロアリ腸内ファージメタゲノム解析と腸内細菌CRISPRスペーサー配列との照合2016

    • Author(s)
      麥島雄太、伊澤和輝、河合幹彦、桑原宏和、雪真弘、大熊盛也、本郷裕一
    • Organizer
      第10回日本ゲノム微生物学会年会
    • Place of Presentation
      東京工業大学(大岡山)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Research-status Report
  • [Presentation] Endomicrobium属細胞内共生細菌の比較ゲノム解析と機能予測2016

    • Author(s)
      伊澤和輝、桑原宏和、木原久美子、Nathan Lo、雪真弘、大熊盛也、本郷裕一
    • Organizer
      第10回日本ゲノム微生物学会年会
    • Place of Presentation
      東京工業大学(大岡山)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Research-status Report
  • [Presentation] Comparative genomics of endomicrobial symbionts associated with distinct protist species in the termite gut2015

    • Author(s)
      Kazuki Izawa, Hirokazu Kuwahara, Kumiko Kihara, Nathan Lo, Masahiro Yuki, Moriya Ohkuma, Yuichi Hongoh
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦
    • Year and Date
      2015-10-18
    • Related Report
      2015 Research-status Report
  • [Presentation] ゲノムから解き明かすシロアリ腸内複合共生系2015

    • Author(s)
      本郷裕一
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-29
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] シロアリ腸内原生生物に細胞表面共生するSymbiothrix dinenymphaeの1細胞ゲノム解析2015

    • Author(s)
      雪昌弘,桑原宏和,新谷政己,伊澤和輝,本郷裕一,大熊盛也
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Bacteroidetes門微生物の窒素固定に関する諸解析2015

    • Author(s)
      井上潤一,本郷裕一,坂本光央,大熊盛也
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Diversity and functions of the ecto- and endosymbionts of cellulolytic protists in the gut of termites2015

    • Author(s)
      Yuichi Hongoh
    • Organizer
      Annual Conference 2015 of the Association for General and Applied Microbiology
    • Place of Presentation
      ドイツ・マールブルク
    • Year and Date
      2015-03-02
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 新規Elusimicrobia門細胞内共生細菌のドラフトゲノム配列取得と比較解析2014

    • Author(s)
      伊澤和輝, 桑原宏和, 木原久美子, 雪真弘,Nathan Lo, 伊藤武彦, 大熊盛也, 本郷裕一
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      浜松
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report
  • [Presentation] シロアリ腸内細菌の網羅的な群集構造解析と比較解析2014

    • Author(s)
      菅谷快斗, 山田明徳, 河内雅人, 松島慶, 井上潤一, 雪真弘, 守屋繁春, 大熊盛也, 本郷裕一
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      浜松
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Single cell genomic insights into Treponema diversity in the gut of the higher termite Nasutitermes takasagoensis2014

    • Author(s)
      David Starns, Masahiro Yuki, Hirokazu Kuwahara, Yuichi Hongoh, Alistair Darby, Moriya Ohkuma
    • Organizer
      環境微生物系学会合同大会201
    • Place of Presentation
      浜松
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Comparative single-cell genomics of diverse treponemes in the gut of Nasutitermes takasagoensis2014

    • Author(s)
      David Starns, Masahiro Yuki, Yuichi Hongoh, Alistair Darby, Moriya Ohkuma
    • Organizer
      15th International Symposium for Microbial Ecology (ISME15)
    • Place of Presentation
      韓国ソウル
    • Year and Date
      2014-08-25 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Single-cell genome sequencing of Rs-N74, an ectosymbiont of termite gut protist2014

    • Author(s)
      Masahiro Yuki, Hirokazu Kuwahara, Masaki Shintani, Yuichi Hongoh, Moriya Ohkuma
    • Organizer
      15th International Symposium for Microbial Ecology (ISME15)
    • Place of Presentation
      韓国ソウル
    • Year and Date
      2014-08-25 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Elusimicrobia門細胞内共生細菌Rs-D17種内比較ゲノム解析2014

    • Author(s)
      伊澤和輝, 桑原宏和, 伊藤武彦, 本郷裕一
    • Organizer
      日本進化学会第16回大阪大会
    • Place of Presentation
      大阪
    • Year and Date
      2014-08-21 – 2014-08-23
    • Related Report
      2014 Research-status Report
  • [Remarks] 本郷研究室ホームページ

    • URL

      http://www.hongoh.bio.titech.ac.jp/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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