| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The burrowing mayfly Ephoron shigae is distributed East Asia. Some populations are bisexual, and others are unisexual. In general, parthenogenetic organisms are often found in harsh environments, such as at high latitudes and altitudes, in xeric as opposed to mesic conditions, in isolated habitats, and at the peripheral regions of the taxon’s distribution range. However, the distributions of bisexual and unisexual populations overlap broadly in their respective geographical ranges. In the analysis of mtDNA (16S, COI), we revealed that unisexual populations were of monophyletic origin and recently differentiated in western Japan. In the nuDNA EF-1a, boule analysis, parthenogenetic strains had two genotypes, while specimens of bisexual lineage had 20 genotypes. These results are consistent with an automixis mode of reproduction for the parthenogenetic strains. Furthermore, there would be no gene flow between the specimens of the bisexual lineage and those of the parthenogenetic strain.
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