Exploration of unknown genetic function in subseafloor biosphere by Substrate-Induced Gene Expression approach

• Project/Area Number: 26650169
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: MORONO Yuki 国立研究開発法人海洋研究開発機構, 高知コア研究所, グループリーダー代理 (30421845)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Subseafloor biosphere / Gene function / 海底下生命圏 / 未知機能遺伝子 / 基質誘導 / 遺伝子発現 / 遺伝子機能 / 海底下 / 未知微生物

Outline of Final Research Achievements

In this study, we aimed to explore genetic function of microbial life inhabited in subseafloor biosphere. For searching unknown microbial genetic function, we employed substrate-induced gene expression (SIGEX) approach that relies gene expression induced by exposure of substrate.

Research Products

• [Journal Article] A modified SDS-based DNA extraction method for high quality environmental DNA from (2016)
  • Author(s): Vengadesh Perumal Natarajan, Xinxu Zhang, Yuki Morono, Fumio Inagaki and Fengping Wang
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 986-986
  • DOI: 10.3389/fmicb.2016.00986
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ecophysiology of Zetaproteobacteria Associated with Shallow Hydrothermal Iron-Oxyhydroxide Deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan (2015)
  • Author(s): HOSHINO TATSUHIKO, Kuratomi Takashi,MORONO YUKI,Hori Tomoyuki,Oiwane Hisashi,Kiyokawa Shoichi,INAGAKI FUMIO
  • Journal Title: Frontiers in Microbiology Volume: 6 Pages: 1554-1554
  • DOI: 10.3389/fmicb.2015.01554
  • Peer Reviewed / Open Access
• [Journal Article] Exploring deep microbial life in coal-bearing sediments down to ~2.5km below the seafloor (2015)
  • Author(s): INAGAKI FUMIO, Kai-Uwe Hinrichs,KUBO YUSUKE,Marshall W. Bowles,Verena B. Heuer,Wei-Li Hong,HOSHINO TATSUHIKO,IJIRI AKIRA,IMACHI HIROYUKI,ITO MOTOO,KANEKO MASANORI,Mark A. Lever,Lin Yu-Shin,Barbara A. Methe,Morita Sumito,MORONO YUKI,et al.
  • Journal Title: SCIENCE/AAAS/ Volume: 349 Issue: 6246 Pages: 420-424
  • DOI: 10.1126/science.aaa6882
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Intact preservation of environmental samples by freezing under an alternating magnetic field (2015)
  • Author(s): Morono, Y., Terada, T., Yamamoto, Y., Xiao, N., Hirose, T., Sugeno, M., Ohwada, N., Inagaki F
  • Journal Title: Environ. Microbiol. Rep. Volume: 7 Issue: 2 Pages: 243-251
  • DOI: 10.1111/1758-2229.12238
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Origins of lithium in submarine mud volcano fluid in the Nankai accretionary wedge (2015)
  • Author(s): Nishio, Y., Ijiri, A., Toki, T., Morono, Y., Tanimizu, M., Nagaishi, K., and Inagaki, F.
  • Journal Title: Earth Plant. Sci. Lett. Volume: 414 Pages: 144-155
  • DOI: 10.1016/j.epsl.2015.01.018
  • Peer Reviewed
• [Journal Article] 海底下環境における微生物生命圏とその生態 (2014)
  • Author(s): 諸野祐樹
  • Journal Title: Journal of Japanese Society for Extremophiles Volume: 13 Pages: 4-10
• [Presentation] Technological breakthroughs in search of the deep subseafloor biosphere (2016)
  • Author(s): 諸野 祐樹、寺田 武志、伊藤 元雄、稲垣 史生
  • Organizer: Japan Geoscience Union Meeting 2016
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2016-05-22
  • Invited
• [Presentation] 海底下に広がる巨大生命圏とその探索ー最先端技術で生命の実態にせまるー (2016)
  • Author(s): 諸野祐樹
  • Organizer: 日本農芸化学会中四国支部創立15周年記念第22回若手研究者シンポジウム
  • Place of Presentation: 高知大学農学部（高知県南国市）
  • Year and Date: 2016-04-23
  • Invited
• [Presentation] 深海底コア試料を用いたSIGEX法によるD-アミノ酸/希少糖代謝酵素遺伝子の 網羅的探索 (2015)
  • Author(s): 添田 愛理沙, 諸野 祐樹,寺田 武志,稲垣 史生,芦内 誠,若松 泰介
  • Organizer: 日本農芸化学会2015年度中四国・西日本支部合同大会
  • Place of Presentation: 愛媛大学 （愛媛県松山市）
  • Year and Date: 2015-09-18
• [Presentation] 深海底コア試料を用いたSIGEX法によるレアメタル応答遺伝子の網羅的探索と 解析 (2015)
  • Author(s): 尾崎 和弘, 諸野 祐樹,寺田 武志,西川 聡美,北村 萌,稲垣 史生,芦内 誠,若松 泰介
  • Organizer: 日本農芸化学会2015年度中四国・西日本支部合同大会
  • Place of Presentation: 愛媛大学 （愛媛県松山市）
  • Year and Date: 2015-09-18
• [Presentation] Technical developments to explore sedimentary biosphere: current status, limitation, and future perspectives (2015)
  • Author(s): MORONO YUKI,
  • Organizer: CDEBI Sediment Workshop
  • Place of Presentation: University of Southern California (USA)
  • Year and Date: 2015-06-25
  • Int'l Joint Research / Invited
• [Presentation] 海底下で太古の生命を探せー最先端技術で迫る海底下深部生命とその姿, (2014)
  • Author(s): 諸野祐樹
  • Organizer: 高知大学自主セミナー
  • Place of Presentation: 高知大学農学部、高知県南国市
  • Year and Date: 2014-11-05
  • Invited
• [Presentation] エアロゾルDNAフリー環境の構築とその評価 (2014)
  • Author(s): 諸野祐樹、星野辰彦、鈴木剛人、佐藤卓広、湯浅久史、久保田裕仁、寺田武志、稲垣史生
  • Organizer: 極限環境生物学会年会
  • Place of Presentation: 今帰仁村コミュニティセンター、沖縄県国頭郡
  • Year and Date: 2014-11-02
• [Presentation] 基質誘導型遺伝子発現解析による海底下微生物の機能遺伝子探索 (2014)
  • Author(s): 諸野祐樹、寺田武志、星野辰彦、二神泰基、稲垣史生
  • Organizer: 環境微生物系学会合同大会
  • Place of Presentation: 浜松アクトシティ、静岡県浜松市
  • Year and Date: 2014-10-24
• [Presentation] Intact preservation of environmental samples by freezing under an alternating magnetic field (2014)
  • Author(s): Morono Y.
  • Organizer: Fourth C-DEBI Annual Meeting
  • Place of Presentation: Marina, California, USA
  • Year and Date: 2014-10-03
  • Invited
• [Presentation] 熱アルカリ細胞溶菌法による海底下微生物群集構造解析 (2014)
  • Author(s): 諸野祐樹、寺田武志、星野辰彦、稲垣史生
  • Organizer: 日本地球惑星科学連合2014年大会
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2014-04-30
• [Presentation] 海底下に広がる生命圏, (2014)
  • Author(s): 諸野祐樹
  • Organizer: 統合国際深海掘削計画10年の成果一般公開シンポジウム、深海を掘る
  • Place of Presentation: 国立科学博物館、東京都台東区
  • Year and Date: 2014-04-06
  • Invited
• [Book] Earth and Life Processes Discovered from Subseafloor Environments: A Decade of Science Achieved by the Integrated Ocean Drilling Program (IODP) (2014)
  • Author(s): Morono, Y., Kallmeyer, J.
  • Total Pages: 325
  • Publisher: Elsevier
• [Book] “Life in Extreme Environments: Microbial Life in the Deep Biosphere”, Chapter 5 (2014)
  • Author(s): Morono, Y., Ito, M., and Inagaki, F.
  • Total Pages: 822
  • Publisher: DeGruyter