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Toward the development of an RNA silencing control system allowing quantitative modification of plant metabolites

Research Project

Project/Area Number 26660001
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Research InstitutionHokkaido University

Principal Investigator

KANAZAWA Akira  北海道大学, 農学研究院, 准教授 (30281794)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords遺伝子発現 / 発現制御 / 植物 / 発生 / RNA / 遺伝子
Outline of Final Research Achievements

Spatial and temporal dynamics of RNA silencing in the life cycle of plants were analyzed in terms of exploiting quantitative modification of plant metabolites through gene expression control mediated by RNA silencing. Using transgenic soybean plants that carry a reporter gene, the following points were revealed: the induction and spread of RNA silencing during plant growth and its reset during reproduction occur in each generation; specific features of the architecture of the plant, in addition to the mechanisms conserved in plants, are involved in the diversity of these phenomena.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Invited: 2 results)

  • [Journal Article] RNA silencing in the life cycle of soybean: multiple restriction systems and spatiotemporal variation associated with plant architecture2017

    • Author(s)
      Mori, A., Sato, H., Kasai, M., Yamada, T. and Kanazawa, A.
    • Journal Title

      Transgenic Res.

      Volume: 26 Issue: 3 Pages: 349-362

    • DOI

      10.1007/s11248-017-0011-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 植物におけるRNAサイレンシングの動態には植物体の構造上の特性が影響する2016

    • Author(s)
      森あゆみ・佐藤 洋・河西めぐみ・山田哲也・金澤 章
    • Organizer
      日本遺伝学会第88回大会
    • Place of Presentation
      日本大学(静岡県、三島市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] レトロトランスポゾンの挿入を介した植物の環境適応とエピミュータジェネシス:重複遺伝子を持つダイズにおける事例2016

    • Author(s)
      金澤 章
    • Organizer
      2016年度国立遺伝学研究所 研究集会「転移因子と宿主の相互作用による生命機能と進化」
    • Place of Presentation
      国立遺伝学研究所(静岡県、三島市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ダイズにおけるレトロトランスポゾンSORE-1 のsmall RNA 産生とDNAメチル化2016

    • Author(s)
      御厨 駿
    • Organizer
      日本育種学会第129回講演会
    • Place of Presentation
      横浜市立大学(神奈川県、横浜市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Research-status Report
  • [Presentation] ペチュニアのカルコン合成酵素遺伝子のコサプレッションとエピジェネティックな表現型の復帰過程におけるシトシンメチル化の動態2015

    • Author(s)
      福本沙弥
    • Organizer
      日本育種学会第128回講演会
    • Place of Presentation
      新潟大学(新潟県、新潟市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] ダイズにおけるRNAサイレンシングによる遺伝子発現制御とその利用2015

    • Author(s)
      金澤 章
    • Organizer
      第8回ダイズ研究会(招待講演)
    • Place of Presentation
      岩手大学(盛岡市)
    • Year and Date
      2015-03-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 遺伝子重複とレトロトランスポゾンの挿入を介したダイズの適応進化2014

    • Author(s)
      金澤 章
    • Organizer
      日本育種学会第56回シンポジウム(シンポジウム・ワークショップ)
    • Place of Presentation
      南九州大学(都城市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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