| Project/Area Number |
26660014
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop production science
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| Research Institution | Nagoya University |
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Principal Investigator |
Yoshida Kumi 名古屋大学, 情報科学研究科, 教授 (90210690)
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| Co-Investigator(Renkei-kenkyūsha) |
TURO Masato 名城大学, 農学部, 准教授 (40410774)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | アジサイ / 酸性土壌耐性 / アルミニウム毒性 / Hydrangea macrophylla / acidic soil / Al-tolerance / ICP / HmVALT / HmPALT1 / HmPALT2 |
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| Outline of Final Research Achievements |
Hydrangea macrohpylla accumulates Al3+ in the sepal vacuoles, which is responsible for blue coloration. This phenomenon would be involved in the Al tolerance of hydrangea in acidic soils. To clarify the mechanism of Al-tolerance, blue-cultivars of hydrangea were grown on a acidic and neutral soils from cuttage stage to fully blooming, then, the composition of organic components, Al content and expression level of genes involving in Al-tolerance were analyzed.
Expression level of HmVALT and Al content in leaf of +Al was higher than that of-Al. It is suggested that expression of genes involving Al-tolerance might be induced by Al-treatment at the early stage. The overexpression of HmVALT in Arabidopsis thaliana conferred Al tolerance, therefore, we cultivated A. thaliana with/without Al until the mature stage, then, the Al content and gene expression levels were analyzed. The growth of wild type (Col-0) was defected while the HmVALT overexpressed plant was grown without any damage.
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