Stidies on aluminum tolerance of hydrangea for developping a new strategy against acidic soils

• Project/Area Number: 26660014
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Crop production science
• Research Institution: Nagoya University
• Principal Investigator: Yoshida Kumi 名古屋大学, 情報科学研究科, 教授 (90210690)
• Co-Investigator(Renkei-kenkyūsha): TURO Masato 名城大学, 農学部, 准教授 (40410774)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: アジサイ / 酸性土壌耐性 / アルミニウム毒性 / Hydrangea macrophylla / acidic soil / Al-tolerance / ICP / HmVALT / HmPALT1 / HmPALT2

Outline of Final Research Achievements

Hydrangea macrohpylla accumulates Al3+ in the sepal vacuoles, which is responsible for blue coloration. This phenomenon would be involved in the Al tolerance of hydrangea in acidic soils. To clarify the mechanism of Al-tolerance, blue-cultivars of hydrangea were grown on a acidic and neutral soils from cuttage stage to fully blooming, then, the composition of organic components, Al content and expression level of genes involving in Al-tolerance were analyzed.
Expression level of HmVALT and Al content in leaf of +Al was higher than that of-Al. It is suggested that expression of genes involving Al-tolerance might be induced by Al-treatment at the early stage. The overexpression of HmVALT in Arabidopsis thaliana conferred Al tolerance, therefore, we cultivated A. thaliana with/without Al until the mature stage, then, the Al content and gene expression levels were analyzed. The growth of wild type (Col-0) was defected while the HmVALT overexpressed plant was grown without any damage.

Research Products

• [Journal Article] Metal-complex Pigment Involved in the Blue Sepal Color Development of Hydrangea. (2015)
  • Author(s): Oyama, K-I., Yamada, T., Ito, D., Kondo, T., Yoshida, K.
  • Journal Title: J. Agric. Food. Chem. Volume: 63 Issue: 35 Pages: 7630-7635
  • DOI: 10.1021/acs.jafc.5b02368
  • Peer Reviewed / Open Access
• [Journal Article] Polyphenols: from plants to a variety of food and nonfood uses. (2015)
  • Author(s): Cheynier, V., Tomas-Barberan, F. A., Yoshida, K.
  • Journal Title: J. Agric. Food. Chem. Volume: 63 Issue: 35 Pages: 7589-7594
  • DOI: 10.1021/acs.jafc.5b01173
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemical mechanism of petal color development of Nemophila menziesii by a metalloanthocyanin, nemophilin. (2015)
  • Author(s): Yoshida, K., Tojo, K., Mori, M., Yamashita, K., Kitahara, S., Noda, M., Uchiyama, S.
  • Journal Title: Tetrahedron Volume: 71 Issue: 48 Pages: 9123-9130
  • DOI: 10.1016/j.tet.2015.10.007
  • Peer Reviewed / Open Access
• [Journal Article] Structure of tecophilin, a tri-caffeoylanthocyanin from the blue petals of Tecophilaea cyanocrocus, and the mechanism of blue color development (2014)
  • Author(s): Mori, M., Ito, D., Miki, N., Kondo, T., Yoshida, K.
  • Journal Title: Tetrahedron Volume: 70 Issue: 45 Pages: 8657-8664
  • DOI: 10.1016/j.tet.2014.09.046
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Efficient and versatile synthesis of 5-O-acylquinic acids with a direct esterification using a p-methoxybenzyl quinate as a key intermediate (2014)
  • Author(s): Oyama, K-I., Watanabe, N., Yamada, T., Suzuki, M., Sekiguchi, Y., Kondo, T., Yoshida, K.
  • Journal Title: Tetrahedron Volume: 00 Issue: 20 Pages: 00-00
  • DOI: 10.1016/j.tet.2014.08.064
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 色素としてのポリフェノール—化学と機能— (2014)
  • Author(s): 吉田久美
  • Journal Title: 日本ポリフェノール学会雑誌 Volume: 3 Pages: 20-30
  • NAID: 40020160846
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] アントシアニンのB環構造系統的な色素増感太陽電池の作製と変換効率 (2016)
  • Author(s): 木村友紀、若宮淳志、吉田 久美
  • Organizer: 日本化学会第96春季年会2016
  • Place of Presentation: 京田辺市
  • Year and Date: 2016-03-24
• [Presentation] Synthesis of 8-alkyl-3-deoxyanthocyanidins. (2016)
  • Author(s): 安藤宏基、尾山 公一、吉田 久美
  • Organizer: 日本化学会第96春季年会2016
  • Place of Presentation: 京田辺市
  • Year and Date: 2016-03-24
• [Presentation] 赤アズキ種皮色素の化学的研究 (2016)
  • Author(s): 市川由樹、小西香織、河合加奈、後藤美樹、近藤忠雄、吉田久美
  • Organizer: 日本化学会第96春季年会2016
  • Place of Presentation: 京田辺市
  • Year and Date: 2016-03-24
• [Presentation] 配糖化フラボノールを経由した多アシル化アントシアニンの合成経路の検討 (2016)
  • Author(s): 橋本眞侑、尾山 公一、近藤忠雄、吉田 久美
  • Organizer: 日本化学会第96春季年会2016
  • Place of Presentation: 京田辺市
  • Year and Date: 2016-03-24
• [Presentation] アジサイ由来のアルミニウムストレスの無毒化に関与する液胞膜局在アクアポリンの構造と機能に関する研究 (2016)
  • Author(s): 伊藤誉明、入江克雅、亀川亜希子、大嶋篤典、藤吉好則、吉田久美
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡市
  • Year and Date: 2016-03-18
• [Presentation] Synthesis of O-methylated flavonoids and the studies on physiological functions of flavonoids (2015)
  • Author(s): Kimura, Y., Kondo, T., Yoshida, K.
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 青色花の発色と金属錯体化学 (2015)
  • Author(s): 吉田久美
  • Organizer: 錯体化学会第65回討論会シンポジウム
  • Place of Presentation: 奈良
  • Year and Date: 2015-09-21
  • Invited
• [Presentation] 5-O-アシル化キナ酸類の効率的合成によるアジサイの青色金属錯体色素の化学構造研究 (2015)
  • Author(s): 尾山 公一、山田 智美、伊藤 大輔、渡邉 紀之、関口 由紀子、 鈴木 昌子、近藤 忠雄、吉田 久美
  • Organizer: 第57回 天然有機化合物討論会
  • Place of Presentation: 横浜市
  • Year and Date: 2015-09-09
• [Presentation] Mechanism of Blue Flower Coloration by Anthocyaninss. (2015)
  • Author(s): Yoshida, K.
  • Organizer: Inaugural Symposium of the Phytochemical Society of Asia 2015
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2015-08-30
  • Int'l Joint Research / Invited
• [Presentation] 青色花色発現の解明−化学と生物の間で− (2015)
  • Author(s): 吉田久美
  • Organizer: 日本農芸化学会中四国市部第19回若手シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2015-05-15
  • Invited
• [Presentation] アルキル化フラボノイド類の合成と色素増感太陽電池への応用. (2015)
  • Author(s): 木村友紀、尾山公一、若宮淳志、近藤忠雄、吉田久美
  • Organizer: 日本化学会第95春季年会2015
  • Place of Presentation: 千葉
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 合成した助色素類を用いたアジサイ青色錯体の構造研究. (2015)
  • Author(s): 山田智美、尾山公一、近藤忠雄、吉田久美
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 未熟黒大豆種皮に含まれるflav-2-en-3-ol3-glucosideの単離とアントシアニンへの変換反応に関する研究. (2015)
  • Author(s): 林英美、吉田久美、近藤忠雄
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] クマリンを合成素子とした3-oxykoaburageninの全合成. (2015)
  • Author(s): 今井祥平、尾山公一、渡邉紀之、吉田久美
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] アジサイのアルミニウム処理による組織のAl量と輸送体の発現量の変動. (2015)
  • Author(s): 田内翔子、大原健史、根岸孝至、津呂正人、吉田久美
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Cheimstry of flower coloration by anthocyanins. (2014)
  • Author(s): Yoshida, K.
  • Organizer: 29th Symposium on Natural Products.
  • Place of Presentation: Kauhsiung, Taiwan
  • Year and Date: 2014-09-27 – 2014-09-28
  • Invited
• [Presentation] Chemical Studies of Nemophilin, a Metalloanthocyanin from Blue Petals of Nemophila menziesii. (2014)
  • Author(s): Tojo, K., Yamashita, K., Kitahara, S., Mori, M., Yoshidam K.
  • Organizer: XXVII International Conference on Polyphenols & 8th Tannin Conference.
  • Place of Presentation: Nagoya, JAPAN
  • Year and Date: 2014-09-02 – 2014-09-06
  • Invited
• [Presentation] Synthesis of 3-deoxyanthocyanins. (2014)
  • Author(s): Oyama, K-I., Kondo, T., Yoshida, K.
  • Organizer: The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference.
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Anthocyanin Chemistry in Flower Color Development. (2014)
  • Author(s): Kondo, T., Oyama, K-I., Yoshida, K.
  • Organizer: The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference.
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Synthetic Studies of Alkylated Flavonoids and their Transformation to the Corresponding Anthocyanidins. (2014)
  • Author(s): Kimura, Y., Oyama, K-I., Kondo, T., Yoshida, K.
  • Organizer: The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference.
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Studies on Biosynthetic Intermediate of Cyanidin 3-glucoside from Seed Coat of Black Soybean. (2014)
  • Author(s): Hayashi, E., Kondo, T., Yoshida, K.
  • Organizer: The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference.
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Study on Divergent Synthesis of Flavan-3-ol type Polyphenos Using Coumarin as a Building Block. (2014)
  • Author(s): Imai, S., Oyama, K-I., Watanabe, N., Yoshida, K.
  • Organizer: The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference.
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Studies on Color Variation and Al-accumulation in Sepals of Hydrangea macrophylla. (2014)
  • Author(s): Tauchi, S., Ohara, T., Negishi, T., Yoshida, K.
  • Organizer: The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference.
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Synthesis of 5-O-Acylquinic Acids Involved in Blue Color Development of Hydrangea Sepals. (2014)
  • Author(s): Yamada, T., Oyama, K-I., Watanabe, N., Suzuki, M., Sekiguchi, Y., Kondo, T., Yoshida, K.
  • Organizer: The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference.
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Remarks] YOSHIDA LABORATORY
  • URL: http://www.info.human.nagoya-u.ac.jp/lab/yoshida/
• [Remarks] YOSHIDA LABORATORY - 名古屋大学
  • URL: http://www.info.human.nagoya-u.ac.jp/lab/yoshida/