Exploring candidate gene related to exhibit water core fruit in Japanese pear 'Housui' using microarray analysis between diploid and tetraploid

• Project/Area Number: 26660020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Ibaraki University
• Principal Investigator: Inoue Eiichi 茨城大学, 農学部, 教授 (90292482)
• Research Collaborator: OGATA NATSUMI 茨城県農業総合センター, 生物工学研究所 ; NISHITANI CHIKAKO 農研機構, 果樹研究所
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ニホンナシ / みつ症 / 生理障害 / 豊水 / 原因遺伝子 / 四倍体 / マイクロアレイ / 糖代謝 / ４倍体 / マイクロアレイ解析 / 定量的PCR

Outline of Final Research Achievements

Water core of fruit is one of physiological disorder in Japanese pear. The disorder is very serious because a Japanese leading variety Housui is susceptible to it. Although the water core is thought a heritable character, their genetic mechanism is unknown. Recently, more sensitivity for water core had identified in Housui tetraploid than their diploid. In this research, we explored the candidate genes related to exhibit water core fruit in Japanese pear 'Housui' using microarray analysis between diploid and tetraploid.
As a result of microarray analysis, some gene involving in fruit ripening, senescence and cell wall metabolism were strongly expressed in tetraploid fruit just before the exhibiting water core symptom. After analyzing more detailed DNA expression, we confirmed that two types of gene were highly expressed in tetraploid fruit before the symptom. We concluded that the candidate gene for sugar metabolism relate to the water core in Japanese pear.

Research Products

• [Int'l Joint Research] ボゴール農科大学(インドネシア)
• [Presentation] ニホンナシ果実の‘みつ症’発生機構に関する研究（第６報） ‘豊水’ 染色体 倍加個体の果実における高いみつ症感受性 (2016)
  • Author(s): 井上栄一・八塚拓・野口尚美・尾形夏海・西谷千佳子・ 喜多晃一・郷内武・石井亮二・山本俊哉・東尾久雄
  • Organizer: 園芸学会
  • Place of Presentation: 東京農業大学農学部厚木キャンパス
  • Year and Date: 2016-03-27
• [Presentation] ニホンナシ果実の‘みつ症’発生機構に関する研究（第７報） 倍数性の異なるニホンナシ‘豊水’の果実 におけるみつ症の発症と成熟・糖代謝に関わる遺伝子の関係 (2016)
  • Author(s): 八塚拓・野口尚美・尾形夏海・喜多晃一・郷内武・石井亮二・ 西谷千佳子・山本俊哉・東尾久雄・井上栄一
  • Organizer: 園芸学会
  • Place of Presentation: 東京農業大学農学部厚木キャンパス
  • Year and Date: 2016-03-27