Analysis of molecular mechanisim of transcription factor PAT2 regulated pathenocarpy

• Project/Area Number: 26660024
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Nagoya University
• Principal Investigator: Mori Hitoshi 名古屋大学, 生命農学研究科, 教授 (20220014)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 単為結果 / 転写因子 / 単為結果性 / Zinc finger homeodomain / トマト

Outline of Final Research Achievements

Transcription factor PAT2 was found the causal gene of parthenocarpy in tomato by NARO Institute of Vegetable and Tea Science. I identified target gene that was regulated by transcription factor PAT2 in order to analyze molecular mechanism of parthenocapy. Pat2 expression was decreased and GA3ox one, that is biosynthesis of active GA, was increased by auxin treatment using quantitative PCR in tomato ovary. Furthermore, Pat2 expression was decreased and GA3ox one was increased during developmental stage of ovary. These results indicated that PAT2 suppress GA3ox transcription.

Research Products

• [Journal Article] The AMOR arabinogalactan sugar chain induces pollen tube competency to respond to ovular guidance (2016)
  • Author(s): Mizukami A., Inatsugi R., Jiao J., Kotake T., Kuwata K., Ootani K, Okuda S., Sankaranarayanan S., Sato Y., Maruyama D., Iwai H., Garenaux E., Sato C., Kitajima K., Tsumuraya Y., Mori H., Yamaguchi J., Itami K., Sasaki N. and Higashiyama T.
  • Journal Title: Curr. Biol. Volume: 26 Issue: 8 Pages: 1091-1097
  • DOI: 10.1016/j.cub.2016.02.040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots (2015)
  • Author(s): Yamauchi T, Shiono K, Nagano M, Fukazawa A, Ando M, Takamure I, Mori H, Nishizawa NK, Kawai-Yamada M, Tsutsumi N, Kato K, Nakazono M
  • Journal Title: Plant Physiology Volume: 169 Issue: 1 Pages: 180-193
  • DOI: 10.1104/pp.15.00106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rare allele of a previously unidentified histone H4 acetyltransferase enhances grain weight, yield, and plant biomass in rice. (2015)
  • Author(s): Song XJ, Kuroha T, Ayano M, Furuta T, Nagai K, Komeda N, Segami S, Miura K, Ogawa D, Kamura T, Suzuki T, Higashiyama T, Yamasaki M, Mori H, Inukai Y, Wu J, Kitano H, Sakakibara H, Jacobsen SE, Ashikari M.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 112 Issue: 1 Pages: 76-81
  • DOI: 10.1073/pnas.1421127112
  • Peer Reviewed / Open Access / Acknowledgement Compliant