| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Ovary of persimmon fruit was observed by light microscope at the blooming stage to obtain the developmental process of tannin cells in the fruit, and it revealed that small tannin cells are firstly appeared at the junction of calyx lobe and ovary, and those are distributed toward upper parts of ovary with cell enlargement. At undevelopmental stages of ovary, i.e. 4 or 3 weeks before blooming, tannin cells are only observed in receptacle. When we tested an efficiency of a new fluorescent dye conjugated with fluorescent dye and gelatin to elucidate localization of condensed tannins in persimmon fruit, it showed a successful possibility if preparation method for sectioning was improved. In addition, high gene expression related to proanthocyanidin biosynthesis was detected in the early sage of fruit development in persimmon.
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