| Project/Area Number |
26660027
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | Kyoto Prefectural University (2015-2016)
Tottori University (2014) |
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Principal Investigator |
ITAI Akihiro 京都府立大学, 生命環境科学研究科, 教授 (10252876)
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| Co-Investigator(Kenkyū-buntansha) |
及川 彰 山形大学, 農学部, 准教授 (50442934)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 果実成熟抑制 / メタボローム解析 / トランスクリプトーム解析 / Tree factor / 師管液 / RNA-seq |
|---|
| Outline of Final Research Achievements |
European pear fruit can’t ripen on the tree. They are harvested at the mature green stage and ripened off the tree. A tree factor that inhibits ripening has been postulated to explain this effect. However, until now, nobody has elucidated its identity. Here, we have investigated the profiles of metabolic substances induced by girdling the fruit peduncle to inhibit phloem transport of the tree factor. And we have searched genes related to tree factor’s effect in European pear by RNA-seq analysis. Total 24 substances showed differences between fruit without girdling and with girdling by metabolome analysis. Also, up-regulated 9 genes and down-regulated 34 genes by girdling treatment were identified by RNA-seq analysis.
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