Analysis on the resistance against plant viruses through the levels of endogenous ascorbic acid
| Project/Area Number |
26660031
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Hokkaido University |
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Principal Investigator |
MASUTA Chikara 北海道大学, (連合)農学研究科(研究院), 教授 (60281854)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ビタミンC / RNAサイレンシング / ウイルス / Arabidopsis / ウイルス抵抗性 / アスコルビン酸 / カブモザイクウイルス / RNAサイレンシングサプレッサー / DHAR |
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| Outline of Final Research Achievements |
We demonstrated that endogenous ascorbic acid (AsA) was closely associated with an antiviral response to Turnip mosaic virus (TuMV) in Brassica rapa and Arabidopsis thaliana. The AsA accumulation was induced by TuMV infection in B. rapa cvs. Aki-masari and Ku-kai 65, that have the resistant gene Rnt1-1. They accumulated about 1.5-fold higher levels of AsA 3 days after inoculation of the avirulent TuMV (strain UK1). Our gene expression analysis suggested that the observed AsA increase was due to the suppression of AsA oxidation by ascorbate peroxidase (APX) and ascorbate oxidase (AO), and the activation of AsA-recycling by dehydroascorbate reductase (DHAR). Endogenously supplied JA and JA derivatives actually increased the endogenous level of AsA. We therefore reasoned that the induction of the AsA accumulation in Aki-masari may be mediated by the JA-dependent signaling pathway as a defense reaction against viruses.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Rice necrosis mosaic virus, a fungal transmistted Bymovirus: complete nucleotide sequence of the genomic RNA 1 and subgrouping of bymoviruses.2015
Author(s)
Wagu, G.S., Kobayashi, K., Yaeno, T., Yamaoka, N., Masuta, C. and Nishiguchi, M.
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Journal Title
Journal of General Plant Pathology
Volume: 82
Pages: 38-42
Related Report
Peer Reviewed
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[Journal Article] The Soybean mycorrhiza-inducible phosphate transporter gene, GmPT7, also shows localized expression at the tips of vein endings of senescent leaves.2014
Author(s)
Inoue Y, Kobae Y, Omoto E, Tanaka A, Banba M, Takai S, Tamura Y, Hirose A, Komatsu K, Otagaki S, Matsumoto S, Taniguchi M, Masuta C, Ishimoto M and Hata S
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Journal Title
Plant and Cell Physiology
Volume: 55
Issue: 12
Pages: 2102-2111
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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