Development of phytoplasma tolerant plants by a novel immunosuppressive therapy

• Project/Area Number: 26660032
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: The University of Tokyo
• Principal Investigator: Himeno Misako 東京大学, 大学院農学生命科学研究科(農学部), 特別研究員 (10646970)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ファイトプラズマ / 篩部壊死 / 病害防除

Outline of Final Research Achievements

Experimental methods to detect phloem cell death caused by phytoplasma infection were investigated, and the optimized method using trypan blue staining was developed. From the results obtained by LC- TOF-MS analysis, phloem cell death caused by phytoplasma infection was suggested to cause impaired phloem loading, resulting in activating anthocyanin biosynthesis pathway. Further, results of inoculation test using mutant plants suggested that anthocyanin accumulation is involved in the reduction of cell death and plant immune response pathway is associated with diseased symptoms. These results may contribute to development of phytoplasma tolerant plants.

Research Products

• [Journal Article] EXA1, a GYF domain protein, is responsible for loss-of-susceptibility to plantago asiatica mosaic virus in Arabidopsis thaliana. (2016)
  • Author(s): Hashimoto M., Neriya Y., Keima T., Iwabuchi N., Koinuma H., Hagiwara-Komoda Y., Ishikawa K., Himeno M., Maejima K., Yamaji Y., Namba S.
  • Journal Title: The Plant Journal Volume: 88 Issue: 1 Pages: 120-131
  • DOI: 10.1111/tpj.13265
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 日本に発生するPPV（ウメ輪紋ウイルス） (2016)
  • Author(s): 前島健作・根津 修・姫野未紗子・難波成任
  • Journal Title: 森林科学 Volume: 76 Pages: 21-26
  • NAID: 110010048557
• [Journal Article] Degradation of class E MADS-domain transcription factors in Arabidopsis by a phytoplasmal effector, phyllogen. (2015)
  • Author(s): Maejima K., Kitazawa Y., Tomomitsu T., Yusa A., Neriya Y., Himeno M., Yamaji Y., Oshima K., Namba S.
  • Journal Title: Plant Signaling & Behavior Volume: 10 Issue: 8 Pages: e1042635-e1042635
  • DOI: 10.1080/15592324.2015.1042635
  • Peer Reviewed / Open Access
• [Journal Article] Functional characterization of the principal sigma factor RpoD of phytoplasmas via an in vitro transcription assay. (2015)
  • Author(s): Miura C., Komatsu K., Maejima K., Nijo T., Kitazawa Y., Tomomitsu T., Yusa A., Himeno M., Oshima K., Namba S.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11893-11893
  • DOI: 10.1038/srep11893
  • Peer Reviewed / Open Access
• [Journal Article] 日本に発生したPPVの特性とその診断 (2015)
  • Author(s): 前島健作・根津 修・姫野未紗子・濱本 宏・難波成任
  • Journal Title: 樹木医学研究 Volume: 19 Pages: 3-6
  • NAID: 130007480945
• [Journal Article] The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways. (2014)
  • Author(s): Minato, N., Himeno, M., Hoshi, A., Maejima, K., Komatsu, K., Takebayashi, Y., Kasahara, H., Yusa, A., Yamaji, Y., Oshima, K., Kamiya, Y. & Namba, S.
  • Journal Title: Scientific reports Volume: 4 Issue: 1 Pages: 1-7
  • DOI: 10.1038/srep07399
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Study on the mechanism of purple top symptoms caused by phytoplasma infection. (2014)
  • Author(s): Himeno M., Kitazawa Y., Yoshida T., Yusa A., Miyazaki A., Neriya Y., Maejima K., Oshima K., Namba S.
  • Journal Title: Japanese Journal of Mycoplasmology Volume: 41 Pages: 2-3
  • Acknowledgement Compliant
• [Journal Article] Onion yellow phytoplasma P38 protein plays a role in adhesion to the hosts. (2014)
  • Author(s): Neriya Y., Maejima K., Nijo T., Tomomitsu T., Yusa A., Himeno M., Netsu O., Hamamoto H., Oshima K., Namba S.
  • Journal Title: FEMS Microbiology Letters Volume: 361 Issue: 2 Pages: 115-122
  • DOI: 10.1111/1574-6968.12620
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Recognition of floral homeotic MADS-domain transcription factors by a phytoplasmal effector, phyllogen, induces phyllody (2014)
  • Author(s): Maejima K., Iwai R., Himeno M., Komatsu K., Kitazawa Y., Fujita N., Ishikawa K., Fukuoka M., Minato N., Yamaji Y., Oshima K., Namba S
  • Journal Title: The Plant J Volume: 78 Issue: 4 Pages: 541-554
  • DOI: 10.1111/tpj.12495
  • Peer Reviewed / Open Access
• [Presentation] ファイトプラズマシグマ因子RpoDを用いたin vitro転写系の確立 (2016)
  • Author(s): 三浦千裕・二條貴通・鯉沼宏章・岩渕望・姫野未紗子・前島健作・小松健・大島研郎・難波成任
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] 酵素標識抗体を用いたdirect tissue stamp 法による感染植物組織内におけるファイトプラズマの所在解析技術の改良 (2016)
  • Author(s): 岩渕望・鯉沼宏章・根津修・笹野百花・姫野未紗子・二條貴通・前島健作・難波成任
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] 鯉沼宏章・岩渕望・根津修・宮崎彰雄・二條貴通・姫野未紗子・前島健作・難波成任 (2016)
  • Author(s): FTAカードを用いたファイトプラズマDNAの長期保存
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] ファイトプラズマ感染植物におけるアントシアニン類の細胞死抑制への関与 (2015)
  • Author(s): 姫野未紗子・遊佐礼・煉谷裕太朗・前島健作・山次康幸・大島研郎・難波成任
  • Organizer: 日本マイコプラズマ学会第42回学術集会
  • Place of Presentation: 慶應義塾大学（東京都港区）
  • Year and Date: 2015-05-22
• [Presentation] パープルトップ症状はファイトプラズマ感染による細胞死の誘導を抑制する (2015)
  • Author(s): 姫野未紗子・友光達哉・遊佐礼・北沢優悟・煉谷裕太朗・前島健作・大島研郎・難波成任
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] ファイトプラズマ感染が引き起こすパープルトップ症状は宿主のアントシアニン生合成経路に依存する (2014)
  • Author(s): 姫野未紗子・北沢優悟・吉田哲也・三浦千裕・前島健作・大島研郎・難波成任
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 北海道
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] ファイトプラズマ感染によるパープルトップ症状誘導メカニズムに関する解析 (2014)
  • Author(s): 姫野未紗子、北沢 優悟、吉田 哲也、遊佐 礼、宮崎 彰雄、煉谷裕太朗、前島健作、大島研郎、難波成任
  • Organizer: 平成26年度日本マイコプラズマ学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-22 – 2014-05-23