| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Experimental methods to detect phloem cell death caused by phytoplasma infection were investigated, and the optimized method using trypan blue staining was developed. From the results obtained by LC- TOF-MS analysis, phloem cell death caused by phytoplasma infection was suggested to cause impaired phloem loading, resulting in activating anthocyanin biosynthesis pathway. Further, results of inoculation test using mutant plants suggested that anthocyanin accumulation is involved in the reduction of cell death and plant immune response pathway is associated with diseased symptoms. These results may contribute to development of phytoplasma tolerant plants.
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