A novely assay of signal transduction on colonization of Ralstonia solanacearum
| Project/Area Number |
26660036
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Kochi University |
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Principal Investigator |
HIKICHI YASUFUMI 高知大学, 教育研究部総合科学系環境医学部門, 教授 (70291507)
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| Co-Investigator(Kenkyū-buntansha) |
KAI Kenji 大阪府立大学, 生命環境科学研究科, 助教 (40508404)
OHNISHI Kouhei 高知大学, 総合科学系, 教授 (50211800)
KIBA Akinori 高知大学, 総合科学系, 教授 (50343314)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 植物細菌 / バイオフィルム / クオラムセンシング / 細胞間隙 / コロニー化 / 病原性 |
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| Outline of Final Research Achievements |
After invading intercellular spaces of roots of tomato plants, Ralstonia solanacearum strain OE1-1 attached on the surfaces of tomato cells vigorously grew there, forming biofilms on tomato cells. The bacterial growth led to activation of the quorum sensing with methyl 3-hydroxymyristate as a QS signal. This led to functioned PhcA, a multi-transcription regulator, which positively regulated not only major extracellular polysaccharide, EPS I, and cell aggregation but also production of a lectin, RS-IIL and secondary metabolites, ralfuranones. RS-IIL was involved in feedback regulation of methyl 3-hydroxymyristate production. Ralfuranones was involved in feedback regulation of PhcA function. PhcA function was involved in biofilm formation which was required for virulence of strain OE1-1. These results indicated that OE1-1 produces mature biofilms on the surfaces of tomato cells after invasion into intercellular spaces, contributing to OE1-1 virulence.
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Report
(3 results)
Research Products
(57 results)
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[Journal Article] SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana.2014
Author(s)
Kiba, A., Galis, I., Hojo, Y., Ohnishi, K., Yoshioka, H., Hikichi, Y.,
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Journal Title
Plos One
Volume: 9
Issue: 5
Pages: e98150-e98150
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Heat shock protein 70 is required for tabtoxinine- B-lactam-induced cell death in Nicotiana benthamiana2014
Author(s)
Ito, M., Yamamoto, Y., Kim, C-S., Ohnishi, K., Hikichia, Y., Kiba, A.
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Journal Title
Journal of Plant Physiology
Volume: 171
Issue: 2
Pages: 173-178
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 細胞間隙へ侵入後の青枯病菌OE1-1 株によるバイオフィルム形成は，methyl 3-hydroxymyristate をシグナルとするクオラムセンシングに依存しており，病原性に必須である2015
Author(s)
曵地康史, 森友花, 石川詩歩, 東本周樹, 大西秀幸, 嶋谷美香, 井上加奈子, 池田健一, 中屋敷均, 木場章範, 大西浩平, 甲斐建次
Organizer
植物微生物研究会第25回研究交流会
Place of Presentation
つくば国際会議場、つくば市
Year and Date
2015-09-14
Related Report
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