| Project/Area Number |
26660036
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Kochi University |
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Principal Investigator |
HIKICHI YASUFUMI 高知大学, 教育研究部総合科学系環境医学部門, 教授 (70291507)
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| Co-Investigator(Kenkyū-buntansha) |
KAI Kenji 大阪府立大学, 生命環境科学研究科, 助教 (40508404)
OHNISHI Kouhei 高知大学, 総合科学系, 教授 (50211800)
KIBA Akinori 高知大学, 総合科学系, 教授 (50343314)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 植物細菌 / バイオフィルム / クオラムセンシング / 細胞間隙 / コロニー化 / 病原性 |
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| Outline of Final Research Achievements |
After invading intercellular spaces of roots of tomato plants, Ralstonia solanacearum strain OE1-1 attached on the surfaces of tomato cells vigorously grew there, forming biofilms on tomato cells. The bacterial growth led to activation of the quorum sensing with methyl 3-hydroxymyristate as a QS signal. This led to functioned PhcA, a multi-transcription regulator, which positively regulated not only major extracellular polysaccharide, EPS I, and cell aggregation but also production of a lectin, RS-IIL and secondary metabolites, ralfuranones. RS-IIL was involved in feedback regulation of methyl 3-hydroxymyristate production. Ralfuranones was involved in feedback regulation of PhcA function. PhcA function was involved in biofilm formation which was required for virulence of strain OE1-1. These results indicated that OE1-1 produces mature biofilms on the surfaces of tomato cells after invasion into intercellular spaces, contributing to OE1-1 virulence.
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