Development of methods for attenuated virus production by genome evolution

• Project/Area Number: 26660038
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Kakizawa Shigeyuki 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (10588669)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ウイルス / 植物 / ゲノム / 植物ウイルス / 進化分子工学

Outline of Final Research Achievements

Rare codons and codon usage frequencies of Arabidopsis thaliana and other several plant species were investigated. Also, these from several plant viruses were investigated. Several strategies were examined to make a transgenic plant that express tRNA genes, and normal transgenic method would be appropriate. Experimental approaches for plant virus infection, genome extraction, and sequencing analysis were tried to be established.