Studies on metabolic pathway involved in oligotrophic growth of heterotrophic bacteria
Project/Area Number |
26660054
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | Tohoku University |
Principal Investigator |
Nagata Yuji 東北大学, 生命科学研究科, 准教授 (30237531)
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Co-Investigator(Kenkyū-buntansha) |
TSUDA MASATAKA 東北大学, 大学院生命科学研究科, 教授 (90172022)
OHTSUBO YOSHIYUKI 東北大学, 大学院生命科学研究科, 助教 (40342761)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 炭酸固定 / oligotroph / スフィンゴモナッド / グリオキシル酸回路 / 次世代型シーケンサー / 次世代シーケンサー |
Outline of Final Research Achievements |
An aerobic and heterotrophic bacterium Sphingobium japonicum UT26 shows oligotrophic growth phenotype, i.e., growth on minimal salt medium without the supply of any carbon source accompanying with the CO2-fixation, by the over expression of the adhX gene. Protein product of adhX showed alcohol dehydrogenase activity, and its activity had equilateral correlation with the oligotrophic phenotype. Furthermore, it was strongly suggested that the adhX-dependent oligotrophic phenotype is one of the bacterial fundamental mechanisms for adaptation to oligotrophic conditions.
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Report
(3 results)
Research Products
(68 results)
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[Journal Article] Complete Genome sequence of a gamma-hexachlorocyclohexane degrader, Sphingobium sp. Strain TKS, which was isolated from a gamma-hexachlorocyclohexane-degrading microbial community2016
Author(s)
Tabata M, Ohhata S, Kawasumi T, Nikawadori Y, Kishida K, Sato T, Ohtsubo Y, Tsuda M, Nagata Y
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Journal Title
Genome Announcements
Volume: 4
Issue: 2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Complete genome sequence of a phenanthrene degrader, Mycobacterium sp. strain EPa45 (NBRC 110737), isolated from a phenanthrene-degrading consortium.2015
Author(s)
Kato H, Ogawa N, Ohtsubo Y, Oshima K, Toyoda A, Mori H, Nagata Y, Kurokawa K, Hattori M, Fujiyama A, Tsuda M
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Journal Title
Genome Announcements
Volume: 3
Issue: 4
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Time-series metagenomic analysis reveals robustness of soil microbiome against chemical disturbance.2015
Author(s)
Kato H, Mori H, Maruyama F, Toyoda A, Oshima K, Endo R, Fuchu G, Miyakoshi M, Dozono A, Ohtsubo Y, Nagata Y, Hattori M, Fujiyama A, Kurokawa K, Tsuda M
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Journal Title
DNA Research
Volume: 22
Issue: 6
Pages: 413-424
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Complete genome sequence of Sphingompyxis macrogoltabidus type strain NBRC 15033, originally isolated as a polyethylene glycol-degrader2015
Author(s)
Ohtsubo Y, Nagata, Y, Numata M, Tsuchikane K, Hosoyama A, Yamazoe A, Tsuda M, Fujita N, Kawai F
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Journal Title
Genome Announcements
Volume: 3
Issue: 6
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Structural and Functional Analysis of Novel Haloalkane Dehalogenase with Two Halide-Binding Sites2014
Author(s)
Chaloupkova R, Prudnikova T, Rezacova P, Prokop Z, Koudelakova T, Daniel L, Brezovsky J, Ikeda-Ohtsubo W, Sato Y, Kuty M, Nagata Y, Smatanova IK, Damborsky J
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Journal Title
Acta Crystallographica Section D
Volume: D70
Issue: 7
Pages: 1884-1897
DOI
Related Report
Peer Reviewed
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[Presentation] Haloalkane dehalogenases in bacteria2015
Author(s)
Yuji Nagata, Ryota Moriuchi, Yoshiyuki Ohtsubo, and Masataka Tsuda
Organizer
The International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
Place of Presentation
Honolulu, Hawaii, USA
Year and Date
2015-12-15
Related Report
Int'l Joint Research / Invited
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[Presentation] リボソームRNA遺伝子の染色体外への移行2015
Author(s)
按田瑞恵, 大坪嘉行, 大久保卓, 菅原雅之, 永田裕二, 津田雅孝, 南澤究, 三井久幸
Organizer
日本遺伝学会第87回大会ワークショップ12「ゲノムとOMICSの比較から現れた遺伝と進化をめぐる驚き」
Place of Presentation
東北大学川内北キャンパス(宮城県、仙台市)
Year and Date
2015-09-24
Related Report
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