| Project/Area Number |
26660055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
熊野 匠人 筑波大学, 生命環境系, 助教 (70585025)
橋本 義輝 筑波大学, 生命環境系, 准教授 (00323254)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 微生物 / 酵素 / 代謝 |
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| Outline of Final Research Achievements |
While enzymes involved in the metabolism of curcumin, a yellow pigment, derived from the rhizomes of a plant (Curcuma longa Linn) were unknown, we previously purified curcumin-converting enzyme from E. coli and characterized. Here, we isolated microorganisms that degrade a curcumin from soil.
From the microorganism exhibiting the highest activity among newly isolated microorganisms, we identified a novel metabolite of curcumin, and found that the metabolite is different from those from E. coli. Based upon the physiological characteristics, the isolated microorganism was identified. We succeeded in the purification of the curcumin-converting enzyme and identification of its enzymatic reaction product.
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