| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Mutations conferring resistance to ribosome-targeting antibiotics and ribosome-targeting antibiotics at subinhibitory concentrations often dramatically alter the phenotypic profile in bacteria. This study has demonstrated that the concept as described above can be applicable to a variety of eukaryotic microorganisms to elicit their potential, such as the ability of fungi Monascus pilosus to produce secondary metabolites，and to alter the phenotypic characteristics of the fission yeast Schizosaccharomyces Japonicus. Interestingly, phenotypic and genetic characterization of the secondary metabolite-overproducing Hygromycin B resistant mutant of M. pilosus revealed that ribosome-targeting antibiotics in eukaryotic microorganisms could have different mechanisms to those in prokaryotic microorganisms for activating their potential.
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