Search for prenyltransferases that accept as a substrate an intermediate of the non-mevalonate pathway and the application of them for polymer synthesis
| Project/Area Number |
26660060
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Nagoya University |
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Principal Investigator |
HEMMI Hisashi 名古屋大学, 生命農学研究科, 准教授 (60302189)
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| Research Collaborator |
OGAWA Takuya 名古屋大学, 大学院生命農学研究科, 大学院生
HAYASHI Yoshifumi 名古屋大学, 大学院生命農学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 酵素 / プレニルトランスフェラーゼ / イソプレノイド / メチルエリスリトールリン酸経路 / メバロン酸経路 / カロテノイド / 非メバロン酸経路 |
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| Outline of Final Research Achievements |
The involvement of a prenyltransferase from Corynebacterium glutamicum in the biosynthesis of C50 carotenoid from a C40 precursor and two molecules of 4-hydroxy 3-methylbutenyl diphosphate (HMBPP) was shown by the introduction of carotenogenic genes into Escherichia coli and by the analysis using an inhibitor. This is the second finding of the enzyme that utilizes HMBPP as a donor substrate. In addition, by reviewing the substrate specificities of various types of prenyltransferases, we found a few enzymes that also accept HMBPP as a donor substrate, and an enzyme that accepts dimethylally diphosphate, which usually acts as a donor substrate, as an acceptor substrate.
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Report
(3 results)
Research Products
(3 results)
