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Search for prenyltransferases that accept as a substrate an intermediate of the non-mevalonate pathway and the application of them for polymer synthesis

Research Project

Project/Area Number 26660060
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionNagoya University

Principal Investigator

HEMMI Hisashi  名古屋大学, 生命農学研究科, 准教授 (60302189)

Research Collaborator OGAWA Takuya  名古屋大学, 大学院生命農学研究科, 大学院生
HAYASHI Yoshifumi  名古屋大学, 大学院生命農学研究科, 大学院生
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords酵素 / プレニルトランスフェラーゼ / イソプレノイド / メチルエリスリトールリン酸経路 / メバロン酸経路 / カロテノイド / 非メバロン酸経路
Outline of Final Research Achievements

The involvement of a prenyltransferase from Corynebacterium glutamicum in the biosynthesis of C50 carotenoid from a C40 precursor and two molecules of 4-hydroxy 3-methylbutenyl diphosphate (HMBPP) was shown by the introduction of carotenogenic genes into Escherichia coli and by the analysis using an inhibitor. This is the second finding of the enzyme that utilizes HMBPP as a donor substrate. In addition, by reviewing the substrate specificities of various types of prenyltransferases, we found a few enzymes that also accept HMBPP as a donor substrate, and an enzyme that accepts dimethylally diphosphate, which usually acts as a donor substrate, as an acceptor substrate.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Presentation (3 results)

  • [Presentation] 好熱性古細菌由来ジホスホメバロン酸デカルボキシラーゼ及びメバロン酸3-キナーゼの変異解析2016

    • Author(s)
      本山賢人、服部 愛、海野英昭、吉村 徹、邊見 久
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Bacteroidetes門バクテリアFlavobacterium johnsoniaeのメバロン酸経路2016

    • Author(s)
      祖父江史明、吉村 徹、邊見 久
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Corynebacterium glutamicum由来C50カロテノイド合成酵素CrtEbのプレニルドナー基質の解明2015

    • Author(s)
      林 佳史、祖父江史明、伊藤智和、吉村 徹、邊見 久
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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