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A novel metal-import system in the pit-forming bacterium and its application for recovery of radioactive compounds and rare metals

Research Project

Project/Area Number 26660062
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

Hashimoto Wataru  京都大学, (連合)農学研究科(研究院), 教授 (30273519)

Co-Investigator(Kenkyū-buntansha) MURATA Kousaku  摂南大学, 理工学部, 教授 (90142299)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords細菌 / アルギン酸 / レアメタル / 輸送体 / 電子顕微鏡 / 原子間力顕微鏡 / X線結晶構造解析 / 示差走査型蛍光定量法 / 金属結合タンパク質 / Sphingomonas属細菌 / 体腔形成細菌
Outline of Final Research Achievements

A gram-negative bacterium, Sphingomonas sp. A1, incorporates metal-chelating alginates. In this study, structure and function of the bacterial cell surface and alginate-binding protein Algp7 were analyzed by microscopy, differential scanning fluorimetry, or X-ray crystallography. The bacterial cells attached to alginate-calcium gels formed a pit on the cell surface. The pit showed about 100 nm in depth and high electrical potential. Algp7 exhibited a capability to bind rare metals such as Sm3+ and Tl3+, and to associate with alginate at the positively charged cluster formed by Lys68 and Lys69. These results suggest that the high electrical potential in the pit contributes to concentration of negatively charged alginate and Algp7 is applicable for recovery of rare metals.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (1 results)

  • [Journal Article] Structure of a bacterial ABC transporter involved in the import of an acidic polysaccharide alginate2015

    • Author(s)
      Yukie Maruyama, Takafumi Itoh, Ai Kaneko, Yu Nishitani, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata
    • Journal Title

      Structure

      Volume: 23 Issue: 9 Pages: 1643-1654

    • DOI

      10.1016/j.str.2015.06.021

    • NAID

      120005627618

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Formation of a single polar flagellum by lateral and polar bacterial flagellar gene sets in Sphingomonas sp. strain A12015

    • Author(s)
      Yukie Maruyama, Masahiro Kobayashi, Kousaku Murata, and Wataru Hashimoto
    • Journal Title

      Microbiology

      Volume: 161 Issue: 8 Pages: 1552-1560

    • DOI

      10.1099/mic.0.000119

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural insights into alginate binding by bacterial cell-surface.2015

    • Author(s)
      Kanate Temtrirath, Kousaku Murata, and Wataru Hasimoto
    • Journal Title

      Carbohydrate Research

      Volume: 404 Pages: 39-45

    • DOI

      10.1016/j.carres.2014.11.008

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism.2015

    • Author(s)
      Takase, R., Mikami, B., Kawai, S., Murata, K., Hashimoto, W.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 48 Pages: 33198-33214

    • DOI

      10.1074/jbc.m114.585661

    • NAID

      120005690199

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alginate-dependent gene expression mechanism in Sphingomonas sp. Strain A1.2014

    • Author(s)
      Chie Hayashi, Ryuichi Takase, Keiko Momma, Yukie Maruyama, Kousaku Murata, and Wataru Hashimoto:
    • Journal Title

      J.Bacteriol

      Volume: 196(14) Issue: 14 Pages: 2691-2700

    • DOI

      10.1128/jb.01666-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 細菌ABCトランスポーターのATP加水分解は、closed型の基質結合タンパク質との相互作用によって惹起される2016

    • Author(s)
      上西加純、金子あい、丸山如江、水野伸宏、馬場清貴、熊坂 崇、三上文三、村田幸作、橋本 渉
    • Organizer
      日本農芸化学会関西支部第493回講演会
    • Place of Presentation
      京都大学楽友会館(京都市左京区)
    • Year and Date
      2016-02-06
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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