| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Cyanobacteria has an alkane biosynthesis pathway that generate alkane and formic acid using intermediates of fatty acid metabolism. In this process, NADPH is converted to NADP+. Because presence of an enzymatic pathway that generate NADPH using the by-product of this reaction, formic acid, was suggested by our genetic experiments, a candidate gene for that reaction was examined. Biochemical analysis of the gene product revealed that the enzymatic reaction performed by this protein was not dependent on formic acid. By further screening genomic library of a cyanobacteria, three novel candidate genes that genetically complement the formate dehydrogenase-deficient phenotype of an E. coli moaD mutant were identified.
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