| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Eicosapentaenoic acid (EPA), one of polyunsaturated fatty acids, has various beneficial effects on human health. EPA is naturally found as an acyl chain of membrane phospholipids and changes the physicochemical properties of the membrane, such as the thickness, elasticity, permeability, and fluidity, to modulate functions of membrane proteins. In this study, we focused on the membrane protein production of an EPA-less mutant of a psychrotrophic EPA-producing bacterium, Shewaenlla livingstonensis Ac10, which is isolated from Antarctic seawater and inducibly produces EPA-containing phospholipids at low temperatures. We found that, in the EPA-less mutant, a specific membrane protein was exported to the extracellular region via membrane vesicle production, suggesting that the defect of EPA facilitates a protein unfolding of specific proteins and induces the protein secretion pathway.
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