Development of genome engineering technology to create genome diversity in yeast
| Project/Area Number |
26660066
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Sojo University (2015)
Osaka University (2014) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Minetaka 大阪大学, 工学研究科, 准教授 (80379130)
SASANO Yu 大阪大学, 工学研究科, 助教 (90640194)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 酵母 / ゲノム工学 / ゲノム編集 / 染色体工学 / ゲノム機能 / 育種 / ゲノムの再編成工学 / ゲノム育種 / 多様性創出工学 |
|---|
| Outline of Final Research Achievements |
We have previously developed chromosome splitting technology (PCS) in the yeast Saccharomyces cerevisiae. It is based on homologous recombination and enables splitting of a chromosome at any point to form two newly derived chromosomes. However, because of low homologous recombination activity, PCS is limited to a single site at a time, which makes the splitting of multiple loci laborious and time-consuming. In this study, we have developed efficient and versatile genome engineering technology named CRISPR-PCS by integrating PCS with the genome editing CRISPR/Cas system. This integration allowed activation of homologous recombination. In fact, the CRISPR-PCS enabled generation of simultaneous multiple chromosome splitting such as generation of up to five derived chromosomes from a single chromosome and eight derived chromosomes from four different chromosomes. The CRISPR-PCS technology should contribute to not only breeding novel yeast strains but also revealing genome function.
|
Report
(3 results)
Research Products
(40 results)
-
-
-
-
-
-
-
-
[Journal Article] Cloning and functional analysis of HpFAD2 and HpFAD3 genes encoding Δ12- and Δ15-fatty acid desaturases in Hansenula polymorpha.2014
Author(s)
Sangwallek, J., Kaneko, Y., Tsukamoto, T., Marui, M., Sugiyama, M., Ono, H., Bamba, T., Fukusaki, E., Harashima, S.
-
Journal Title
Gene
Volume: 533
Issue: 1
Pages: 110-118
DOI
Related Report
Peer Reviewed
-
[Journal Article] Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Δmsg5Δ double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy12014
Author(s)
Lavina, W., Shahsavarani, H., Saidi, A., Sugiyama, M., Kaneko, Y., Harashima, S.
-
Journal Title
J. Biosci. Bioeng
Volume: 117
Issue: 2
Pages: 135-141
DOI
NAID
Related Report
Peer Reviewed
-
[Journal Article] Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae.2014
Author(s)
Sugiyama, M., Akase, SP., Nakanishi, R., Horie, H., Kaneko, Y., Harashima, S.
-
Journal Title
Appl. Environ. Microbiol
Volume: 80
Issue: 11
Pages: 3488-3495
DOI
Related Report
Peer Reviewed
-
[Journal Article] Genome-wide mapping of unexplored essential regions in the Saccharomyces cerevisiae genome: evidence for hidden synthetic lethal combinations in a genetic interaction network.2014
Author(s)
Kaboli, S., Yamakawa, T., Sunada, K., Takagaki, T., Sasano, Y., Sugiyama, M., Kaneko, Y., Harashima, S.
-
Journal Title
Nucleic Acids Res
Volume: 42
Issue: 15
Pages: 9838-9853
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
