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Functional analysis of physiological function-unknown or novel quinoprotein to seek an enzyme generating reduced PQQ

Research Project

Project/Area Number 26660068
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionYamaguchi University

Principal Investigator

Matsushita Kazunobu  山口大学, 創成科学研究科, 教授(特命) (50107736)

Co-Investigator(Kenkyū-buntansha) 片岡 尚也  山口大学, 創成科学研究科, 助教 (50713509)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsPQQ / キノプロテイン / sGDH / グリセロール脱水素酵素 / Acinetobacter / 酢酸菌 / 還元型PQQ / Glycerol Dehydrogenase / 酸化ストレス
Outline of Final Research Achievements

In this study, the effect of radical scavengers was examined on the enzyme activities of Acinetobacter soluble quinoprotein, glucose dehydrogenase, of which the physiological role is still unknown, and also of Gluconobacter membrane-bound quinoprotein, glycerol dehydrogenase, from which the mechanism of PQQ release has not been solved.
Together with examination of the radical generation ability of cofactor PQQ and/or electron acceptors, we have proposed one speculation that these quinoproteins are able to release PQQH2 from the protein at alkaline pH and thus to generate O2-.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (29 results)

All 2017 2016 2015 2014

All Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 11 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (16 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations2016

    • Author(s)
      Matsushita K, Azuma Y, Kosaka T, Yakushi T, Hoshida H, Akada R, Yamada M
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 80(4) Issue: 4 Pages: 655-668

    • DOI

      10.1080/09168451.2015.1104235

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, <i>Acetobacter pasteurianus</i> SKU1108, Provide a New Insight into Thermotolerance2016

    • Author(s)
      Matsutani, M., Hirakawa, H., Hiraoka, E., Theeragool, G., Yakushi, T. and Matsushita, K.
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 4 Pages: 395-400

    • DOI

      10.1264/jsme2.ME16023

    • NAID

      130005434990

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Efficient production of 2,5-diketo-D-gluconate via heterologous expression of 2-keto-D-gluconate dehydrogenase in Gluconobacter japonicus.2015

    • Author(s)
      Kataoka N, Matsutani M, Yakushi T, Matsushita K.
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 81(10) Issue: 10 Pages: 3552-3560

    • DOI

      10.1128/aem.04176-14

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A functionally critical single nucleotide polymorphism in the gene encoding the membrane-bound alcohol dehydrogenase found in ethanol oxidation-deficient Gluconobacter thailandicus2015

    • Author(s)
      P Charoenyingcharoen, M Matsutani, T Yakushi, G Theeragool, P Yukphan, K Matsushita
    • Journal Title

      Gene

      Volume: in press Issue: 2 Pages: 3552-3560

    • DOI

      10.1016/j.gene.2015.04.080

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adaptive mutation related to cellulose producibility in Komagataeibacter medellinensis (Gluconacetobacter xylinus) NBRC 3288.2015

    • Author(s)
      Matsutani M, Ito K, Azuma Y, Ogino H, Shirai M, Yakushi T, Matsushita K.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 99 Issue: 17 Pages: 7229-7240

    • DOI

      10.1007/s00253-015-6598-x

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis.2015

    • Author(s)
      Soemphol W, Tatsuno M, Okada T, Matsutani M, Kataoka N, Yakushi T, Matsushita K.
    • Journal Title

      J Biotechnol.

      Volume: 211 Pages: 46-55

    • DOI

      10.1016/j.jbiotec.2015.06.397

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complete genome and gene expression analyses of Asaia bogorensis reveal unique response to culture with mammalian cells as a potential opportunistic human pathogen2015

    • Author(s)
      Kawai M, Higashiura N, Hayasaki K, Okamoto N, Takami A, Hirakawa H, Matsushita K, Azuma Y
    • Journal Title

      DNA Res

      Volume: 22(5) Issue: 5 Pages: 357-366

    • DOI

      10.1093/dnares/dsv018

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Acetic acid bacteria: A group of bacteria with versatile biotechnological applications.2015

    • Author(s)
      Saichana N, Matsushita K, Adachi O, Frebort I, Frebortova J.
    • Journal Title

      Biotechnol Adv.

      Volume: in press Issue: 6 Pages: 1260-1271

    • DOI

      10.1016/j.biotechadv.2014.12.001

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Replacement of a terminal cytochrome c oxidase by ubiquinol oxidase during the evolution of acetic acid bacteria2014

    • Author(s)
      Matsutani M, Fukushima K, Kayama C, Arimitsu M, Hirakawa H, Toyama H, Adachi O, Yakushi T, Matsushita K.
    • Journal Title

      Biochim Biophys Acta

      Volume: 1837 Issue: 10 Pages: 1810-1820

    • DOI

      10.1016/j.bbabio.2014.05.355

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Draft genome sequence of Gluconobacter thailandicus NBRC 3257.2014

    • Author(s)
      Minenosuke Matsutani, Haruo Suzuki, Toshiharu Yakushi, Kazunobu Matsushita.
    • Journal Title

      Standards in Genomic Sciences

      Volume: 9 Pages: 614-623

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overexpression of a type II 3-dehydroquinate dehydratase enhances the biotransformation of quinate to 3-dehydroshikimate in Gluconobacter oxydans2014

    • Author(s)
      Nishikura-Imamura S, Matsutani M, Insomphun C, Vangnai AS, Toyama H, Yakushi T, Abe T, Adachi O, Matsushita K.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 98 Issue: 7 Pages: 2955-2963

    • DOI

      10.1007/s00253-013-5439-z

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 実験室進化・適応育種に基づく耐熱性機構の解析と高温発酵系の開発2017

    • Author(s)
      松下一信
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Gluconobacter属酢酸菌キノプロテイン・グリセロール脱水素酵素における新たな基質の発見とその反応メカニズムの提唱2017

    • Author(s)
      寺田 優花,尾崎 聖士朗,片岡 尚也,足立 収生,赤壁 善彦,藥師 寿治,松下 一信
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Gluconobacter 属酢酸菌における膜結合型グリセロール脱水素酵素の過剰発現2017

    • Author(s)
      尾崎 聖士朗,鳥飼敬弘,松谷 峰之介,片岡 尚也,藥師 寿治,松下 一信
    • Organizer
      日本農芸化学会中四国支部第47回講演会(例会)
    • Place of Presentation
      島根大学(島根県松江市)
    • Year and Date
      2017-01-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Gluconobacter 属酢酸菌のキノプロテイン・グリセロール脱水素酵素における新しい基質特異性と反応メカニズムの提唱2016

    • Author(s)
      寺田優花, 尾崎聖士朗, 片岡尚也, 足立収生, 赤壁善彦, 薬師寿治, 松下一信
    • Organizer
      日本農芸化学会2016年度中四国支部大会(46回講演会)
    • Place of Presentation
      高知大学(高知県高知市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Gluconobacter frateurii CHM43の耐熱化に関する多剤耐性輸送体の機能解析2016

    • Author(s)
      松本奈実、服部浩美、松谷峰之介、外山博英、片岡尚也、薬師寿治、松下一信
    • Organizer
      日本農芸化学会2016年度年会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] Acetobacter pasteurianus SKU1108株の高温適応化株TH-3の発現・機能解析から見える耐熱性機構2016

    • Author(s)
      松下一信,松谷峰之介,西倉慎顕,秦野智之,サイチャナナツラン,マスードウライワン,服部浩美,貝沼(岡本)章子,兼崎 友,石川森夫,片岡尚也,薬師寿治
    • Organizer
      日本農芸化学会2016年度年会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 酢酸菌Acetobacter pasteurianus 12株のゲノム情報と耐熱性との相関に関する解析2016

    • Author(s)
      松谷峰之介,山下隆司,古川藍子,辰野真木,貝沼(岡本)章子,石川森夫,志波優,吉川博文,平川英樹,薬師寿治,松下一信
    • Organizer
      第10回日本ゲノム微生物学会年会
    • Place of Presentation
      東工大(東京都目黒区)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 酢酸菌の耐熱性に関わる新規Na+ (K+) /H+ antiporter・NhaK2の構造と機能2015

    • Author(s)
      古川藍子,辰野真木,松谷峰之介,Wichai Soemphol*, 片岡尚也,薬師寿治, 松下一信
    • Organizer
      日本生体エネルギー研究会第41回研究会
    • Place of Presentation
      東大医学部(東京都文京区)
    • Year and Date
      2015-12-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 酢酸菌Acetobacter pasteurianus IFO 3283株の高温適応育種と耐熱化遺伝子解析2015

    • Author(s)
      松本奈実、松谷峰之介、東慶直、薬師寿治、松下一信
    • Organizer
      酢酸菌研究会第7回研究集会
    • Place of Presentation
      東京大学農学部(東京都文京区)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 耐熱性酢酸菌Acetobacter pasteurianus SKU1108 (NBRC 101655) 株の完全長ゲノム配列の決定および比較ゲノム解析2015

    • Author(s)
      松谷峰之介、平川英樹、薬師寿治、松下一信
    • Organizer
      酢酸菌研究会第7回研究集会
    • Place of Presentation
      東京大学農学部(東京都文京区)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Research-status Report
  • [Presentation] Characterization the cytoplasmic membrane proteases involved in high temperature growth of thermotolerant Acetobacter tropicalis SKU11002015

    • Author(s)
      Wichai Soemphol, Minenosuke Matsutani, Naoya Kataoka, Toshiharu Yakushi, Hirohide Toyama, Kazunobu Matsushita
    • Organizer
      International Conference on Fermentation Technology for Value Added Agricultural Products with joint sessions from JSPS - NRCT New Core to Core Program Advance Research Networks
    • Place of Presentation
      Centara Hotel & Convention Center, Khon Kaen, Thailand
    • Year and Date
      2015-07-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 2-ケト-D-グルコン酸脱水素酵素の異種発現によるGluconobacter japonicusでの効率的2,5-ジケト-D-グルコン酸生産2015

    • Author(s)
      片岡 尚也、松谷 峰之介、藥師 寿治、松下 一信
    • Organizer
      日本農芸化学会 2015年度大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 酢酸菌Gluconobacter oxydansの膜結合型キナ酸脱水素酵素の高発現2015

    • Author(s)
      小松 和貴、吉原 望、今村 志穂実、片岡 尚也、薬師 寿治、足立 収生、松下 一信
    • Organizer
      日本農芸化学会 2015年度大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 酢酸菌Acetobacter pasteurianus SKU1108の持つ2種類の膜結合型アルデヒド脱水素酵素の機能解析2015

    • Author(s)
      新納 俊、福成 聖也、児玉 知大、松谷 峰之介、片岡 尚也、薬師 寿治、Gunjana Theeragool、松下 一信
    • Organizer
      日本農芸化学会 2015年度大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 高温適応育種された酢酸菌Acetobacter pasteurianusの遺伝子工学的変異解析2015

    • Author(s)
      谷本 陽子、西倉 慎顕、松谷 峰之介、片岡 尚也、Gunjana Theeragool、薬師 寿治、松下 一信
    • Organizer
      日本農芸化学会 2015年度大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 酢酸菌Komagataeibacter medellinensis NBRC 3288株のセルロース非生産株から生産株への復帰機構の解明2015

    • Author(s)
      松谷 峰之介、伊藤 光平、東 慶直、荻野 英賢、白井 睦訓、薬師 寿治、松下 一信
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学六甲第2キャンパス(神戸市)
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Research-status Report
  • [Book] Acetic acid bacteria: Ecology and Physiology2016

    • Author(s)
      Kazunobu Matsushita, Hirohide Toyama, Naoto Tonouchi, Akiko Okamoto-Kainuma (Editors)
    • Publisher
      Springer Japan
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 耐熱性微生物の作製方法2014

    • Inventor(s)
      赤田倫治,星田尚司,山田守,村田正之,高坂智之,松下一信,他3名
    • Industrial Property Rights Holder
      山口大学・近畿大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-159048
    • Filing Date
      2014-08-04
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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