| Project/Area Number |
26660068
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
片岡 尚也 山口大学, 創成科学研究科, 助教 (50713509)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | PQQ / キノプロテイン / sGDH / グリセロール脱水素酵素 / Acinetobacter / 酢酸菌 / 還元型PQQ / Glycerol Dehydrogenase / 酸化ストレス |
|---|
| Outline of Final Research Achievements |
In this study, the effect of radical scavengers was examined on the enzyme activities of Acinetobacter soluble quinoprotein, glucose dehydrogenase, of which the physiological role is still unknown, and also of Gluconobacter membrane-bound quinoprotein, glycerol dehydrogenase, from which the mechanism of PQQ release has not been solved.
Together with examination of the radical generation ability of cofactor PQQ and/or electron acceptors, we have proposed one speculation that these quinoproteins are able to release PQQH2 from the protein at alkaline pH and thus to generate O2-.
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