Development of Liquid Isolation protocol of microorganisms in a mixed culture system by using fluoro-micromanipulator

• Project/Area Number: 26660069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyushu University
• Principal Investigator: Sakai Kenji 九州大学, (連合)農学研究科(研究院), 教授 (50205704)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 蛍光顕微マニピュレーター / 難培養微生物 / 高度好熱菌 / 難培養微生物分離 / 顕微蛍光マニピュレーター / 複合微生物系 / マニピュレーション / 蛍光顕微鏡

Outline of Final Research Achievements

In this study, efficient liquid isolation protocol of not-yet isolated bacteria using fluoro-micromanipulator was developed. Type of cqpilally, plate, and oil as well as position of duple, washing, and application of FDA (reagent for detecting viable cells), observation of micro-colonies were optimized. As a results, two types of interesting microorganisms showing exteme-thermophilicity were successfully purified from high-temperature compost.

Research Products

• [Journal Article] Unique hyper-thermal composting process in Kagoshima City forms distinct bacterial community structures (2016)
  • Author(s): Yukihiro Tashiro, Hanae Tabata, Asuka Itahara, Natsuki Shimizu, Kosuke Tashiro, Kenji Sakai
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 122
  • NAID: 40021011747
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Direct starch fermentation to L-lactic acid by a newly isolated thermophilic strain, Bacillus sp. MC-07 (2015)
  • Author(s): Pramod Poudel、田代幸寛、宮本浩邦、宮本久、奥川友紀、酒井謙二
  • Journal Title: Journal of Industrial Microbiology & Biotechnology Volume: 42 Issue: 1 Pages: 143-149
  • DOI: 10.1007/s10295-014-1534-0
  • Peer Reviewed / Open Access
• [Journal Article] Isolation of thermophilic L-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition (2014)
  • Author(s): Saowanit Tongpim、 Ratchanu Meoydong、Poudel Pramod、吉野諭、奥川友紀、田代幸寛、谷口正之、酒井謙二
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 117 Issue: 3 Pages: 318-324
  • DOI: 10.1016/j.jbiosc.2013.08.017
  • NAID: 110009815383
  • Peer Reviewed / Open Access
• [Journal Article] Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost (2014)
  • Author(s): Pramod Poudel、宮本浩邦、宮本久、奥川友紀、田代幸寛、酒井謙二
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Volume: 64 Issue: Pt_8 Pages: 2668-2674
  • DOI: 10.1099/ijs.0.059329-0
  • Peer Reviewed / Open Access
• [Presentation] Exploring Thermotolerants and Thermophiles in Waste Biomass Transformation (2015)
  • Author(s): Kenji Sakai
  • Organizer: FerVAAP2015
  • Place of Presentation: コンケン（タイ）
  • Year and Date: 2015-07-26
  • Int'l Joint Research / Invited
• [Presentation] Mixed culture system for sustainable utilization of food waste biomass to produce value added products under high temperature (2014)
  • Author(s): Pramod Poudel, 田代幸寛, 酒井謙二
  • Organizer: The 8th International Symposium on the East Asian Environmental Problems
  • Place of Presentation: Fukuoka
  • Year and Date: 2014-12-09 – 2014-12-10
• [Book] 高水分バイオマスの液肥利用 第2章 高水分バイオマスの資源リサイクルの重要性と液肥化処理における微生物学的側面 (2014)
  • Author(s): 酒井謙二
  • Total Pages: 336
  • Publisher: 筑波書房