Development of Liquid Isolation protocol of microorganisms in a mixed culture system by using fluoro-micromanipulator
| Project/Area Number |
26660069
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
Sakai Kenji 九州大学, (連合)農学研究科(研究院), 教授 (50205704)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 蛍光顕微マニピュレーター / 難培養微生物 / 高度好熱菌 / 難培養微生物分離 / 顕微蛍光マニピュレーター / 複合微生物系 / マニピュレーション / 蛍光顕微鏡 |
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| Outline of Final Research Achievements |
In this study, efficient liquid isolation protocol of not-yet isolated bacteria using fluoro-micromanipulator was developed. Type of cqpilally, plate, and oil as well as position of duple, washing, and application of FDA (reagent for detecting viable cells), observation of micro-colonies were optimized. As a results, two types of interesting microorganisms showing exteme-thermophilicity were successfully purified from high-temperature compost.
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Report
(3 results)
Research Products
(7 results)
