Epigenom analysis of multipotent cells by Lactic Acid Bacteria

• Project/Area Number: 26660070
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Oita University (2015-2016); Kumamoto University (2014)
• Principal Investigator: Kawano Rie 大分大学, 医学部, 病院特任助教 (20468002)
• Co-Investigator(Renkei-kenkyūsha): OHTA Kunimasa 熊本大学, 生命科学研究部神経分化学講座, 准教授 (90244128) ; NAOFUMI Ito 熊本大学, 生命科学研究部神経分化学講座, 博士研究員
• Research Collaborator: Abdulhaleem F. ; MIHOKO Imori
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 乳酸菌 / リプログラミング / 分化誘導 / 乳酸菌誘導多能性幹細胞 / 多分化能 / 増殖能 / 山中因子 / エピジェネティック

Outline of Final Research Achievements

The lactic acid bacterium induced pluripotent stem cell has ability to differentiate to 3 germ layers (endoblast, mesoderm, ectoderm), like iPS cells or ES cell. We analyzed gene expression using the real-time RT-PCR method when we derived a human fibroblast to multipotent stem cells using a lactic acid bacterium. The lactic acid bacterium induced multipotent stem cell strongly induces the expression of NANOG gene in comparison with iPS cells, ES cells. The NANOG gene product is necessary to maintain the undifferentiated ability of the cell. The possibility that high expression of NANOG gene causes a drop of the increase ability of the lactic acid bacterium induced multipotent stem cell is suggested.
In addition, we are searching among lactic acid bacterium cell body ingredients now what is necessary for undifferentiated ability.

Research Products

• [Journal Article] Akihirin regulates the proliferation and differentiation of neural stem cells in intact and injured mouse spinal cord (2015)
  • Author(s): 1.Abdulhaleen M. F.A., Song X., Kawano R., Uezono N., Ito A., Ahmed G., Hossain M., Nakashima K., Tanaka H., Ohta, K.
  • Journal Title: Dev. Neurobio. Volume: 75 Issue: 5 Pages: 494-504
  • DOI: 10.1002/dneu.22238
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Tsukushi inhibits myofibroblast differentiation by competing with TGF-beta１ (2016)
  • Author(s): Rie Kawano, Shinya Takakura, Daisuke Niimori, Jun-ichi Kadota,Kunimasa Ohta
  • Organizer: 日本再生医療学会 総会
  • Place of Presentation: 大阪国際会議場（大阪府 大阪市）
  • Year and Date: 2016-03-19
• [Presentation] Tsukushi inhibits myofibroblast differentiation by competing with TGF-beta１ (2015)
  • Author(s): Rie Kawano, Shinya Takakura, Daisuke Niimori, Jun-ichi Kadota,Kunimasa Ohta
  • Organizer: International symposium on chronic inflammatory disease, Kumamoto
  • Place of Presentation: 熊本大学 薬学部（熊本県 熊本市）
  • Year and Date: 2015-10-16
  • Int'l Joint Research