• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Epigenom analysis of multipotent cells by Lactic Acid Bacteria

Research Project

Project/Area Number 26660070
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionOita University (2015-2016)
Kumamoto University (2014)

Principal Investigator

Kawano Rie  大分大学, 医学部, 病院特任助教 (20468002)

Co-Investigator(Renkei-kenkyūsha) OHTA Kunimasa  熊本大学, 生命科学研究部神経分化学講座, 准教授 (90244128)
NAOFUMI Ito  熊本大学, 生命科学研究部神経分化学講座, 博士研究員
Research Collaborator Abdulhaleem F.  
MIHOKO Imori  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords乳酸菌 / リプログラミング / 分化誘導 / 乳酸菌誘導多能性幹細胞 / 多分化能 / 増殖能 / 山中因子 / エピジェネティック
Outline of Final Research Achievements

The lactic acid bacterium induced pluripotent stem cell has ability to differentiate to 3 germ layers (endoblast, mesoderm, ectoderm), like iPS cells or ES cell. We analyzed gene expression using the real-time RT-PCR method when we derived a human fibroblast to multipotent stem cells using a lactic acid bacterium. The lactic acid bacterium induced multipotent stem cell strongly induces the expression of NANOG gene in comparison with iPS cells, ES cells. The NANOG gene product is necessary to maintain the undifferentiated ability of the cell. The possibility that high expression of NANOG gene causes a drop of the increase ability of the lactic acid bacterium induced multipotent stem cell is suggested.
In addition, we are searching among lactic acid bacterium cell body ingredients now what is necessary for undifferentiated ability.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Akihirin regulates the proliferation and differentiation of neural stem cells in intact and injured mouse spinal cord2015

    • Author(s)
      1.Abdulhaleen M. F.A., Song X., Kawano R., Uezono N., Ito A., Ahmed G., Hossain M., Nakashima K., Tanaka H., Ohta, K.
    • Journal Title

      Dev. Neurobio.

      Volume: 75 Issue: 5 Pages: 494-504

    • DOI

      10.1002/dneu.22238

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Tsukushi inhibits myofibroblast differentiation by competing with TGF-beta12016

    • Author(s)
      Rie Kawano, Shinya Takakura, Daisuke Niimori, Jun-ichi Kadota,Kunimasa Ohta
    • Organizer
      日本再生医療学会 総会
    • Place of Presentation
      大阪国際会議場(大阪府 大阪市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Tsukushi inhibits myofibroblast differentiation by competing with TGF-beta12015

    • Author(s)
      Rie Kawano, Shinya Takakura, Daisuke Niimori, Jun-ichi Kadota,Kunimasa Ohta
    • Organizer
      International symposium on chronic inflammatory disease, Kumamoto
    • Place of Presentation
      熊本大学 薬学部(熊本県 熊本市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi