Visualization, sorting, and transcriptome analysis of Escherichia coli subpopulations with different generations of poles.
| Project/Area Number |
26660078
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SUYAMA Tetsushi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (10357311)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Takafumi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (40344163)
NODA Naohiro 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究グループリーダー (70415727)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 大腸菌 / 細胞分裂 / サブポピュレーション / 蛍光蛋白質 / 光変換 / LED / バイオイメージング / 分取 / 極 / 融合蛋白質 / 標識 / 可視化 |
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| Outline of Final Research Achievements |
Rod-shaped bacteria (e.g., Escherichia coli) are the gathering of subpopulations with multi-generations of their older poles and their physiological traits are different to each other. In this study, we constructed an E. coli with a photoconvertible fluorescent protein conjugated to a chemoreceptor protein Tar localized to their poles, and the generations of poles were successfully visualized. We have further investigated the method of sorting, and established a protocol of photo-labeling without damaging the cells. Specific weight of the cells was found to be a promising method for differentiating the subpopulations with different generations of poles. We have also composed an automatic cell-sorting system utilizing a microfluidic chip, a fluorescent microscope, and a photonic multichannel analyzer for collecting more variety of subpopulations to clarify the mechanisms of modulating growth rate and entering VBNC state in the subsequent studies.
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Report
(3 results)
Research Products
(2 results)
