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Visualization, sorting, and transcriptome analysis of Escherichia coli subpopulations with different generations of poles.

Research Project

Project/Area Number 26660078
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

SUYAMA Tetsushi  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (10357311)

Co-Investigator(Kenkyū-buntansha) MIZUNO Takafumi  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (40344163)
NODA Naohiro  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究グループリーダー (70415727)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords大腸菌 / 細胞分裂 / サブポピュレーション / 蛍光蛋白質 / 光変換 / LED / バイオイメージング / 分取 / 極 / 融合蛋白質 / 標識 / 可視化
Outline of Final Research Achievements

Rod-shaped bacteria (e.g., Escherichia coli) are the gathering of subpopulations with multi-generations of their older poles and their physiological traits are different to each other. In this study, we constructed an E. coli with a photoconvertible fluorescent protein conjugated to a chemoreceptor protein Tar localized to their poles, and the generations of poles were successfully visualized. We have further investigated the method of sorting, and established a protocol of photo-labeling without damaging the cells. Specific weight of the cells was found to be a promising method for differentiating the subpopulations with different generations of poles. We have also composed an automatic cell-sorting system utilizing a microfluidic chip, a fluorescent microscope, and a photonic multichannel analyzer for collecting more variety of subpopulations to clarify the mechanisms of modulating growth rate and entering VBNC state in the subsequent studies.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2016 2015

All Presentation (2 results)

  • [Presentation] Conditions for Photoconversion and Characterization of Escherichia coli Strains Labeled with Photoconvertible Fluorescent Proteins2016

    • Author(s)
      陶山哲志, 水野敬文
    • Organizer
      日本農芸化学会2016年度札幌大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光変換型蛍光蛋白質Kaedeを用いた大腸菌の細胞分裂と極齢の観察2015

    • Author(s)
      陶山哲志 水野敬文
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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