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Novel mechanism of starch-granule degradation and its application

Research Project

Project/Area Number 26660079
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionHokkaido University

Principal Investigator

KIMURA Atsuo  北海道大学, (連合)農学研究科(研究院), 教授 (90186312)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords植物 / デンプン粒分解 / 長鎖基質認識酵素 / 澱粉粒分解 / 酵素
Outline of Final Research Achievements

This project aims at the formation of theory that plant α-glucosidase is the key-enzyme to degrade starch granules by elucidating its molecular mechanism. The following results were obtained. (1) Plant enzymes display the starch granule-binding and -hydrolyzing abilities, implying that key-enzyme is an α-glucosidase. (2) We clarify the long substrate recognition mechanism of sugar beet α-glucosidase by the analysis using its three-dimensional structure and long substrate-mimic inhibitors. (3) We also reveal the structural factor of rice enzyme to bind and hydrolyze starch granules. (4) It is found that plant β-amylase and α-glucosidase synergistically degrade starch granules.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] 長鎖阻害剤の利用による植物α-グルコシダーゼの機能構造相関の解明2016

    • Author(s)
      田上貴祥, 山下恵太郎, 奥山正幸, 森 春英, 姚 閔, 木村淳夫
    • Journal Title

      応用糖質科学

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] α-Glucosidases and α-1,4-glucan lyases: structures, functions, and physiological actions2016

    • Author(s)
      Masayuki Okuyama, Wataru Saburi, Haruhide Mori, and Atsuo Kimura
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural advantage of sugar beet α-glucosidase to stabilize the Michaelis complex with long-chain substrate2015

    • Author(s)
      Tagami T, Yamashita K, Okuyama M, Mori H, Yao M, Kimura A
    • Journal Title

      J. Biol. Chem.

      Volume: 290 Issue: 3 Pages: 1796-1803

    • DOI

      10.1074/jbc.m114.606939

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 長鎖阻害剤の利用による植物α-グルコシダーゼの機能構造相関の解明2015

    • Author(s)
      田上貴祥、山下恵太郎、奥山正幸、森春英、姚閔、木村淳夫
    • Organizer
      応用糖質科学シンポジウム
    • Place of Presentation
      東大寺総合文化センター(奈良県奈良市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Structural and biochemical studies of sugar beet α-glucosidase exhibiting high specificity for long-chain substrates.2015

    • Author(s)
      Takayoshi Tagami, Keitaro Yamashita, Masayuki Okuyama, Haruhide Mori, Min Yao, Atsuo Kimura
    • Organizer
      11th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      Otaniemi (Finland)
    • Year and Date
      2015-05-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] テンサイα-グルコシダーゼの高重合度基質特異性はらせん構造の長鎖基質に適したサブサイト構造に起因する2015

    • Author(s)
      田上貴祥, 山下恵太郎, 奥山正幸, 森 春英, 姚 閔, 木村淳夫
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学(岡山県・岡山市)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Book] Application of Microbiology and Biochemistry. in "Frontiers of Agricultural Science"2015

    • Author(s)
      Atsuo Kimura, Masayuki Okuyama
    • Total Pages
      277
    • Publisher
      Shoukadoh Book Sellers
    • Related Report
      2014 Research-status Report
  • [Remarks] 分子酵素学研究室

    • URL

      http://www.agr.hokudai.ac.jp/rfoa/abs/abs2-3.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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