Novel mechanism of starch-granule degradation and its application
| Project/Area Number |
26660079
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIMURA Atsuo 北海道大学, (連合)農学研究科(研究院), 教授 (90186312)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 植物 / デンプン粒分解 / 長鎖基質認識酵素 / 澱粉粒分解 / 酵素 |
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| Outline of Final Research Achievements |
This project aims at the formation of theory that plant α-glucosidase is the key-enzyme to degrade starch granules by elucidating its molecular mechanism. The following results were obtained. (1) Plant enzymes display the starch granule-binding and -hydrolyzing abilities, implying that key-enzyme is an α-glucosidase. (2) We clarify the long substrate recognition mechanism of sugar beet α-glucosidase by the analysis using its three-dimensional structure and long substrate-mimic inhibitors. (3) We also reveal the structural factor of rice enzyme to bind and hydrolyze starch granules. (4) It is found that plant β-amylase and α-glucosidase synergistically degrade starch granules.
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Report
(3 results)
Research Products
(8 results)
