| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Microbial synthetic system for lactate (LA)-based polymer, as an advanced member of microbial polyester (polyhydroxyalkanoate) family, was already established by development of lactate-polymerizing enzyme. In order to regulate the monomer composition in P(LA-co-3-hydroxybutyrate (3HB)) copolymer, I have applied evolutionary engineering to NADPH-dependent acetoacetyl-CoA reducatase (PhaB) through the plate assay-based enzyme evolution program. As the result, I obtained two beneficial PhaB enzymes with increased activities that can also increased microbial polymer production. Acquired beneficial properties of these evolved P(3HB) enzymes can be accounted for by flexibility increase in the substrate-related region based on the tertiary structure bearing two domains.
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