| Project/Area Number |
26660082
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Ueda Takuya 東京大学, 新領域創成科学研究科, 教授 (80184927)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | バクテリオロドプシン / ＡＴＰ合成酵素 / 光合成 / ATP合成酵素 / リポソーム / PURE system / 膜蛋白質 |
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| Outline of Final Research Achievements |
For production of membrane protein, liposome integrated PURE system was developed. Using this system, bacteriorhodopsin and FoF1-ATP synthase were successfully expressed onto lipid bilayer. While bacteriorhodopsin expressed onto liposome composed of soy bean extract exhibited proton-pumping activities, artificial liposome such as POPC cannot provided scaffold for the activity of bacteriorhodopsin. Concerning FoF1-ATP synthase, amount of template DNA for individual subunit was optimized for the synthesis of active ATPase. Both synthesized bacteriorhodopsin and ATPase were evaluated by measuring enzymatic activities. To synthesize photosynthetic cell, the Gigantic Unilamellar Vesicle (GUV) containing the Small Unilamellar Vesicles (SUV) onto which bacteriorhodopsin and FoF1-ATP synthase were translocated, were developed.
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