| Project/Area Number |
26660083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Fushinobu Shinya 東京大学, 大学院農学生命科学研究科(農学部), 教授 (00302589)
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| Research Collaborator |
FUJITA Kiyotaka 鹿児島大学, 農学部, 准教授
ISHIWATA Akihiro 理化学研究所, 専任研究員
ITO Yukishige 理化学研究所, 主任研究員
Beckham Gregg T. 米国NREL, 研究員
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | グリコシダーゼ / シロイヌナズナ / イネ / β-アラビノオリゴ糖 / 糖質関連酵素 |
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| Outline of Final Research Achievements |
We initially aimed to study the structure and function of uncharacterized enzyme from plants that belong to DUF1680. Although we examined heterologous expression of the genes from Arabidopsis thaliana and Oryza sativa under various conditions, they did not express or expressed as insoluble aggregates. We determined a novel crystal structure of a GH146 β-L-arabinofuranosidase from plant pathogen, Xanthomonas. The active site of the Xanthomonas enzyme was cysteine, like the case of GH127 enzyme (HypBA1), and the enzyme had a novel carbohydrate-binding module domain.
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