Research Project
Grant-in-Aid for Challenging Exploratory Research
Light-dependent protochlorophyllide reductase (LPOR), which requires light for catalysis, operates in chlorophyll biosynthesis. LPOR belongs to the short chain dehydrogenase (SDR) family and has an intrinsic loop structure that provides the structural basis for the light-driven catalytic function in addition to the common structure of the SDR family. In this study, we tried to re-create a new LPOR from a library constructed using synthetic semi-random DNA in the cyanobacterial mutant, in which shows a lethal phenotype under high light conditions in Synechocystis sp. PCC 6803. However, at the moment, a mutant carrying such a new LPOR has not yet isolated. We consider two main technical hurdles that should be solved in future; 1) semi-random DNA fragments have length variation (±2 bases) in current synthetic technology. 2) the parental strain contains a cell population bearing high-light tolerant revertants, which may be generated by high mutation rate in the recJ mutant.
All 2017 2016 2015
All Journal Article (6 results) (of which Peer Reviewed: 6 results, Open Access: 5 results, Acknowledgement Compliant: 4 results) Presentation (1 results)
The Journal of General and Applied Microbiology
Volume: 63 Issue: 2 Pages: 131-138
10.2323/jgam.2016.08.004
130006770752
Biochemical and Biophysical Research Communications
Volume: 470 Issue: 3 Pages: 704-709
10.1016/j.bbrc.2016.01.070
Bio-Protocol
Volume: 5
Volume: 463 Issue: 4 Pages: 1328-1333
10.1016/j.bbrc.2015.06.124
Plant and Cell Physiology
Volume: 57 Issue: 2 Pages: 334-345
10.1093/pcp/pcu165
Life
Volume: 5 Issue: 2 Pages: 1172-1203
10.3390/life5021172
