Establishment of the new strategy for accurate diagnosis of zinc deficiency
| Project/Area Number |
26660086
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Taiho Kambe 京都大学, 生命科学研究科, 准教授 (90303875)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 亜鉛欠乏 / 診断法 / 健康増進 / 超高齢社会 / 亜鉛要求性酵素 |
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| Outline of Final Research Achievements |
In general, zinc deficiency is diagnosed by evaluation of serum zinc status. However, serum zinc status is altered by daily fluctuation or during inflammation, which may be a problem in the case that the accurate diagnosis is required for elderly people because they often suffer from various chronic diseases. Because, at present, Japan faces super-aged society at present, establishing the new strategy for accurate diagnosis of zinc deficiency is more important than ever.
In this study, I tried to establish such strategy by generating useful monoclonal antibody to detect proteins/enzymes whose expression is changed in response to zinc status. Moreover, I investigated zinc requiring enzyme activity whose activity was changed during zinc deficiency. I generated one antibody, which nicely detects zinc transporter in zinc-deficient dependent pattern, and one enzyme whose activity shows drastic change in response to zinc deficiency.
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Report
(3 results)
Research Products
(42 results)
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[Journal Article] Novel mutations in SLC30A2 involved in the pathogenesis of transient neonatal zinc deficiency2016
Author(s)
Itsumura N, Kibihara Y, Fukue K, Miyata A, Fukushima K, Tamagawa-Mineoka R, Katoh N, Nishito Y, Ishida R, Narita H, Kodama H, Kambe T
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Journal Title
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Properties of Zip4 accumulation during zinc deficiency and its usefulness to evaluate zinc status: a study of the effects of zinc deficiency during lactation2016
Author(s)
Hashimoto A, Nakagawa M, Tsujimura N, Miyazaki S, Kizu K, Goto T, Komatsu Y, Matsunaga A, Shirakawa H, Narita H, Kambe T, Komai M
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Journal Title
Am J Physiol Regul Integr Comp Physiol
Volume: 310
Issue: 5
Pages: R459-R468
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Ethambutol neutralizes lysosomes and causes lysosomal zinc accumulation.2015
Author(s)
Yamada D, Saiki S, Furuya N, Ishikawa K, Imamichi Y, Kambe T, Fujimura T, Ueno T, Koike M, Sumiyoshi K, Hattori N
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Journal Title
Biochem Biophys Res Commun.
Volume: 471
Issue: 1
Pages: 109-116
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: a potential novel strategy to enhance zinc absorption by ZIP4 targeting2015
Author(s)
2)Hashimoto, A., Ohkura, K., Takahashi, M., Kizu, K., Narita, H., Enomoto, S., Miyamae, Y., Masuda, S., Nagao, M., Irie, K., Ohigashi, H., Andrews, G. K., Kambe, T.
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Journal Title
Biochemical Journal
Volume: 472
Issue: 2
Pages: 183-193
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Altered expression of two zinc transporters, SLC30A5 and SLC30A6, underlies a mammary gland disorder of reduced zinc secretion into milk.2015
Author(s)
Kumar L, Michalczyk A, McKay J, Ford D, Kambe T, Hudek L, Varigios G, Taylor PE, Ackland ML.
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Journal Title
Genes Nutr.
Volume: 10
Issue: 5
Pages: 487-487
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis2015
Author(s)
Kaneko M, Noguchi T, Ikegami S, Sakurai T, Kakita A, Toyoshima Y, Kambe T, Yamada M, Inden M, Hara H, Oyanagi K, Inuzuka T, Takahashi H, Hozumi I.
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Journal Title
J Neurosci Res
Volume: 93(2)
Issue: 2
Pages: 370-9
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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