Establishment of the new strategy for accurate diagnosis of zinc deficiency
| Project/Area Number |
26660086
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Taiho Kambe 京都大学, 生命科学研究科, 准教授 (90303875)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 亜鉛欠乏 / 診断法 / 健康増進 / 超高齢社会 / 亜鉛要求性酵素 |
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| Outline of Final Research Achievements |
In general, zinc deficiency is diagnosed by evaluation of serum zinc status. However, serum zinc status is altered by daily fluctuation or during inflammation, which may be a problem in the case that the accurate diagnosis is required for elderly people because they often suffer from various chronic diseases. Because, at present, Japan faces super-aged society at present, establishing the new strategy for accurate diagnosis of zinc deficiency is more important than ever.
In this study, I tried to establish such strategy by generating useful monoclonal antibody to detect proteins/enzymes whose expression is changed in response to zinc status. Moreover, I investigated zinc requiring enzyme activity whose activity was changed during zinc deficiency. I generated one antibody, which nicely detects zinc transporter in zinc-deficient dependent pattern, and one enzyme whose activity shows drastic change in response to zinc deficiency.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] Novel mutations in SLC30A2 involved in the pathogenesis of transient neonatal zinc deficiency2016
Author(s)
Itsumura N, Kibihara Y, Fukue K, Miyata A, Fukushima K, Tamagawa-Mineoka R, Katoh N, Nishito Y, Ishida R, Narita H, Kodama H, Kambe T
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Journal Title
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Genome-wide analyses in neuronal cells reveal that upstream transcription factors regulate lysosomal gene expression2016
Author(s)
Yamada D, Saiki S, Furuya N, Ishikawa K, Imamichi Y, Kambe T, Fujimura T, Ueno T, Koike M, Sumiyoshi K, Hattori N
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Journal Title
FEBS JOURNAL
Volume: 471
Pages: 1077-1087
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Properties of Zip4 accumulation during zinc deficiency and its usefulness to evaluate zinc status: a study of the effects of zinc deficiency during lactation.2016
Author(s)
Hashimoto A, Nakagawa M, Tsujimura N, Miyazaki S, Kizu K, Goto T, Komatsu Y, Matsunaga A, Shirakawa H, Narita H, Kambe T, Komai M
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Journal Title
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology
Volume: 310
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: a potential novel strategy to enhance zinc absorption by ZIP4 targeting.2015
Author(s)
2)Hashimoto, A., Ohkura, K., Takahashi, M., Kizu, K., Narita, H., Enomoto, S., Miyamae, Y., Masuda, S., Nagao, M., Irie, K., Ohigashi, H., Andrews, G. K., Kambe, T.
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Journal Title
Biochem J
Volume: 472
Pages: 183-193
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Altered expression of two zinc transporters, SLC30A5 and SLC30A6, underlies a mammary gland disorder of reduced zinc secretion into milk.2015
Author(s)
Kumar L, Michalczyk A, McKay J, Ford D, Kambe T, Hudek L, Varigios G, Taylor PE, Ackland ML.
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Journal Title
Genes Nutr.
Volume: 10
Pages: 487-487
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Zinc transporters ZnT3 and 6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis.2015
Author(s)
Kaneko M, Noguchi T, Ikegami S, Sakurai T, Kakita A, Toyoshima Y, Kambe T, Yamada M, Inden M, Hara H, Oyanagi K, Inuzuka T, Takahashi H, Hozumi I.
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Journal Title
Journal of Neuroscience Reseach
Volume: 93(2)
Pages: 6-11
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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