THE ESTABLISHMENT OF HIGH-THROUGHPUT ANALYSIS SYSTEM OF MULTIPLE ENZYME ACTIVITIES FOR THE PROMOTION OF NUTRI-OMICS STUDY.
Project/Area Number |
26660107
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Food science
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Research Institution | The University of Tokyo |
Principal Investigator |
Kato Hisanori 東京大学, 総括プロジェクト機構, 教授 (40211164)
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Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 酵素活性 / 一斉分析 / 網羅解析 / 食品 / ハイスループット |
Outline of Final Research Achievements |
In nutrition study, it is essential to capture the metabolic profile at the level of enzyme activity. However, the measurement of multiple enzyme activity is generally time-consuming and requires a lot of work. Therefore we aimed to establish an easy and rapid method to analyze multiple metabolic enzyme activities using a high-throughput spectrophotometer. Our experiment in rats under feeding/fasting conditions showed that multiple metabolic enzyme activities (at least 16 enzymes) can be measured by rapid and automated method using the high-throughput spectrophotometer. Also, we showed the differential response between the changes of enzyme activity and gene expression.
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Report
(3 results)
Research Products
(2 results)