• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

THE ESTABLISHMENT OF HIGH-THROUGHPUT ANALYSIS SYSTEM OF MULTIPLE ENZYME ACTIVITIES FOR THE PROMOTION OF NUTRI-OMICS STUDY.

Research Project

Project/Area Number 26660107
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionThe University of Tokyo

Principal Investigator

Kato Hisanori  東京大学, 総括プロジェクト機構, 教授 (40211164)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords酵素活性 / 一斉分析 / 網羅解析 / 食品 / ハイスループット
Outline of Final Research Achievements

In nutrition study, it is essential to capture the metabolic profile at the level of enzyme activity. However, the measurement of multiple enzyme activity is generally time-consuming and requires a lot of work. Therefore we aimed to establish an easy and rapid method to analyze multiple metabolic enzyme activities using a high-throughput spectrophotometer. Our experiment in rats under feeding/fasting conditions showed that multiple metabolic enzyme activities (at least 16 enzymes) can be measured by rapid and automated method using the high-throughput spectrophotometer. Also, we showed the differential response between the changes of enzyme activity and gene expression.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2015

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] The Establishment of High-Throughput Analysis System of Multiple Enzyme Activities for2015

    • Author(s)
      Kenji Saito and Hisanori Kato
    • Organizer
      12th Asian Congress of Nutrition
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-05-17
    • Related Report
      2014 Research-status Report
  • [Presentation] The Establishment of High-Throughput Analysis System of Multiple Enzyme Activities for the Promotion of Nutri-Omics Study2015

    • Author(s)
      Saito K. and Kato H.
    • Organizer
      12th Asian Congress of Nutrition, JAPAN (2015)
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi