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Formation of nanostructure of food plant proteins

Research Project

Project/Area Number 26660111
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionKyoto University

Principal Investigator

URADE REIKO  京都大学, (連合)農学研究科(研究院), 教授 (90167289)

Co-Investigator(Kenkyū-buntansha) SUGIYAMA Masaaki  京都大学, 原子炉実験所, 教授 (10253395)
SATO Nobuhiro  京都大学, 原子炉実験所, 助教 (10303918)
Co-Investigator(Renkei-kenkyūsha) OBA Yojiro  京都大学, 原子炉実験所, 助教 (60566793)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsSAXS / SANS / gliadin / glutenin / beta-conglycinin / glycinin / rheological properties / nanostructure / グリアジン / グルテニン / ナノ構造 / β‐コングリシニン / グリシニン / 動的粘弾性 / 量子ビーム小角散乱 / コムギ
Outline of Final Research Achievements

In this study, nanoscale structures of hydrated gliadins were investigated primarily by SAXS, USAXS and SANS over a wide range of concentrations. Gliadins are soluble in distilled water below 10 wt %. Guinier analyses of SAXS profiles indicate that gliadins are present as monomers together with small amounts of dimers and oligomers in a very dilute solution. Above 15 wt %, gliadins form gel-like hydrated solids. At greater concentrations, a steep upturn appears in the low-q region owing to the formation of large aggregates, and a broad shoulder appears in the middle-q region showing density fluctuation inside. NaCl shortens the distance between nanometer-scale aggregates and causes the formation of larger aggregates. These changes in nanostructure are well correlated with changes in dynamic viscoelasticity of hydrated gliadins.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Molecular assembly of wheat gliadins into nanostructures: A small- angle X‑ray scattering study of gliadins in distilled water over a wide concentration range.2016

    • Author(s)
      Sato, N., Matsumiya, A., Higashino, Y., Funaki, S., Kitao, Y., Oba, Y., Inoue, R., Arisaka, F., Sugiyama, M., Urade, R.
    • Journal Title

      J. Agric. Food Chem.

      Volume: 63 Pages: 8715-8721

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression and characterization of protein disulfide isomerase family proteins in bread wheat2015

    • Author(s)
      Shizuka Kimura, Yuki Higashino, Yuki Kitao, Taro Masuda and Reiko Urade
    • Journal Title

      BMC Plant Biology

      Volume: 15 Issue: 1 Pages: 73-73

    • DOI

      10.1186/s12870-015-0460-2

    • NAID

      120005947146

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Disulfide bond formation activity of soybean quiescin sulfhydryl oxidase2014

    • Author(s)
      Aya Okuda, Motonori Matsusaki, Yuki Higashino, Taro Masuda and Reiko Urade
    • Journal Title

      FEBS Journal

      Volume: 281 Issue: 23 Pages: 5341-5355

    • DOI

      10.1111/febs.13079

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 超小角X線散乱法による小麦タンパク質グリアジン水和凝集体の構造解析2016

    • Author(s)
      佐藤信浩、東野 ゆうき、北尾 悠樹、岩村 紗季、岡田 ひかり、奥田 綾、松崎 元紀、大場 洋次郎、井上 倫太郎、杉山 正明、裏出 令子
    • Organizer
      農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 分画グリアジンを用いたグリアジン分子種間相互作用の解析2016

    • Author(s)
      北尾 悠樹、東野 ゆうき、岩村 紗季、裏出 令子
    • Organizer
      農芸化学会2016年度大会
    • Place of Presentation
      札幌子コンベンションセンター
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] NANOSTRUCTURE OF WHEAT PROTEIN GLIADIN EXTRACTED IN WATER2015

    • Author(s)
      N. Sato., R. Uradeb, Y. Higashinob, S. Funakib, Y. Kitaob, Y. Obaa, R. Inouea, M. Sugiyamaa
    • Organizer
      16Th International Conference on small-angle scattering
    • Place of Presentation
      Berlin
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] グルテニンの物性とナノ構造にたいする塩化ナトリウムの影響2015

    • Author(s)
      東野ゆうき、船木智史、佐藤 信浩、杉山 正明、大場 洋次郎、井上 倫太郎、北尾 悠樹、裏出 令子
    • Organizer
      2015年日本農芸化学会岡山大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Remarks]

    • URL

      http://www.hinshitsusekkei.kais.kyoto-u.ac.jp/

    • Related Report
      2015 Annual Research Report
  • [Remarks] コムギ粉の食品加工特性とコムギ種子貯蔵タンパク質の構造との関係

    • URL

      http://www.hinshitsusekkei.kais.kyoto-u.ac.jp/research04.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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