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Development of mutation clock using Sasa kurilensis

Research Project

Project/Area Number 26660129
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Forest science
Research InstitutionAkita Prefectural University

Principal Investigator

Inoue Mizuki  秋田県立大学, 生物資源科学部, 助教 (80432342)

Co-Investigator(Kenkyū-buntansha) MAKITA Akifumi  秋田県立大学, 生物資源科学部, 教授 (60315596)
SUYAMA Yoshihisa  東北大学, 大学院農学研究科, 准教授 (60282315)
Suzuki Junichiro  首都大学東京, 理工学研究科, 准教授 (00291237)
Co-Investigator(Renkei-kenkyūsha) Saito Tomoyuki  独立行政法人森林総合研究所, 森林植生研究領域, 研究員 (00414483)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
KeywordsNGS / ササ / GBS / SNPs / 齢 / クローナル植物 / 次世代シーケンサー / 体細胞突然変異 / ジェネット / コアレセント理論 / Mutation Clock / 寿命
Outline of Final Research Achievements

We tried to get genome-wide SNPs using modified GBS and developed new method to estimate the longevity. We used 24 leaves of Sasa kurilensis in Towada, whose longevity has already identified by flowering interval (18years old: each 4 ramets of 2 genet and 8 ramets of 1genet , 80years old: 8 ramets of 1genet). We found no difference in numbers of SNPs among new and old individuals. However, we found the diversity of SNPs was higher in old individuals than in younger ones. It suggested that information from SNPs using NGS might be useful to estimate longevity.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2016

All Presentation (1 results)

  • [Presentation] NGSを利用したチシマザサのジェネット内SNPs変異2016

    • Author(s)
      岡野邦宏・松尾歩・蒔田明史 ・鈴木準一郎・ 陶山佳久・井上みずき
    • Organizer
      第63回日本生態学会
    • Place of Presentation
      仙台
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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