The development of transparent method of formalin-fixed ovary specimen of fish

• Project/Area Number: 26660160
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Kyushu University
• Principal Investigator: Matsuyama Michiya 九州大学, (連合)農学研究科(研究院), 教授 (00183955)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 小型浮魚 / 卵巣標本 / バッチ産卵数 / 透明化 / マサバ / マイワシ / マアジ / ホルマリン標本 / 成熟度 / 魚類資源 / 成熟度調査 / ホルマリン固定 / 透明化法

Outline of Final Research Achievements

The objective of the present study is 1) to develop a method of clearing the formalin-fixed ovary specimens of small pelagic fish such as sardine, horse mackerel, and chub mackerel, and 2) to establish a method to estimate the batch fecundity of the fish using the transparent ovary specimens.
Formalin-fixed ovarian specimens of the three marine fish were treated with SeeDB solution mainly composed of fructose, and we revealed the optimum conditions for conveniently transparent. Further, measuring the number of nuclear migrating eggs in the ovarian samples of jack mackerel enabled to calculate the batch fecundity without using a hydrated eggs.

Research Products

• [Journal Article] Characterization of gonadotropin-releasing hormone and gonadotropin in jack mackerel (Trachurus japonicus) : Comparative gene expression analysis with respect to reproductive dysfunction in captive and wild fish. (2014)
  • Author(s): Yui Imanaga, Mitsuo Nyuji, et al.
  • Journal Title: Aquaculture Volume: 428-429 Pages: 226-235
  • DOI: 10.1016/j.aquaculture.2014.03.003
  • Peer Reviewed
• [Presentation] マイワシの産卵誘導実験系と仔稚魚の育成 (2015)
  • Author(s): 松山倫也，田口伸剛，五十嵐修吾，田和篤史，米田道夫，田中寛繁，山口明彦，北野 載，長野直樹
  • Organizer: 2015年度水産海洋学会研究発表大会
  • Place of Presentation: 釧路市
  • Year and Date: 2015-10-09
• [Presentation] アジ、サバ、イワシの繁殖生理 (2015)
  • Author(s): 松山倫也
  • Organizer: 九州大学システム生命科学府システム生命夏の学校
  • Place of Presentation: 福岡市
  • Year and Date: 2015-06-28
  • Invited
• [Presentation] ホルマリン固定卵の簡易透明化法 －マアジ卵を用いたBA-BBとSeeDBの比較－ (2014)
  • Author(s): 杉原直樹，入路光雄，依田真里，酒井猛、北野 載，山口明彦，松山倫也
  • Organizer: 2014年度水産海洋学会研究発表大会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-14 – 2014-11-17
• [Presentation] マアジ受精卵の孵化時間および仔稚魚の成長に水温が及ぼす影響 (2014)
  • Author(s): 魚住和弘，酒井 猛，高橋素光，依田真里，松山倫也
  • Organizer: 平成26年度日本水産増殖学会第13回大会
  • Place of Presentation: 東広島市
  • Year and Date: 2014-10-18 – 2014-10-19
• [Presentation] 卵生産に必要なエネルギーはいつ獲得されるのか？－炭素・窒素安定同位体比に基づく小型浮魚類での検証－ (2014)
  • Author(s): 13.田中寛繁，米田道夫，北野載，長野直樹，川村耕平，田和篤史，松山倫也，大下誠二
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] 飼育下におけるマイワシの産卵誘導および孵化仔魚の成長過程 (2014)
  • Author(s): 16.田口伸剛，田和篤史，山口明彦，長野直樹，北野載，松山倫也
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] ホルマリン固定したマアジ卵巣標本の簡易透明化法 (2014)
  • Author(s): 21.杉原直樹，入路光雄，依田真里，酒井猛，北野載，山口明彦，松山倫也
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-09-19 – 2014-09-22
• [Book] Chapater 4: Egg Production, 109 pp. In “HANDBOOK OF APPLIED FISHERIES REPRODUCTIVE BIOLOGY FOR STOCK ASSESSMENT AND MANAGEMENT” (2014)
  • Author(s): Ganias K, Murua H, Claramunt G, Dominguez-Petit R, Gonçalves P, Juanes F, Keneddy J, Klibansky N, Korta M, Kurita Y, Lowerre-Barbieri S, Macchi G., Matsuyama M, Medina A, Nunes C, Plaza G, Rideout R, Somarakis S, Thorsen A, Uriarte A, Yoneda M.
  • Total Pages: 109
  • Publisher: Digital CSIC. http://hdl.handle.net/10261/87768