Development of method for investigation of feeding habit of marine organisms during early life stages: application to ecological research
| Project/Area Number |
26660162
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
Kodama Keita 国立研究開発法人国立環境研究所, 環境リスク研究センター, 主任研究員 (90391101)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 食性解析 / ペプチド核酸 / 底棲魚介類 / 初期生活史 / 東京湾 / 食性 / 生活史初期 / 甲殻類 |
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| Outline of Final Research Achievements |
We developed a method of PCR-based diet analysis using the peptide nucleic acid (PNA) as a blocking probe for inhibiting DNA amplification of mantis shrimp from Tokyo Bay. We designed universal primers to amplify 28S rDNA for aquatic organisms of a wide range of taxon, and also designed a PNA probe specific to mantis shrimp 28S rDNA. We analyzed DNA template of host (mantis shrimp) and non-host organisms with the universal primers and PNA probe, and confirmed that inhibition of host DNA amplification. PNA-based analysis on samples including stomach and adjacent tissues (e.g., carapace and pleopod) of larvae and juveniles detected potential prey organisms, such as copepods and polychaetes. However, we also detected sequences of non-prey organisms, such as ctenophores whose mucus was attached to the external surface of the host organism. Further modification is required to minimize the effect of non-host contamination, and achieve more efficient detection of prey organisms.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Temporal trends for inflow of perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) to Tokyo Bay, Japan, estimated by a receptor-oriented approach2015
Author(s)
①Sakurai T, Serizawa S, Isobe T, Kobayashi J, Kodama K, Lee JH, Maki H, Zushi Y, Sevilla-Nastor JB, Imaizumi Y, Suzuki N, Horiguchi T, Shiraishi H
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Journal Title
Sci. Total Environ.
Volume: 539
Pages: 277-285
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 東京湾へのPFOSおよびPFOAの流入速度の経年変化（2004-2010）2015
Author(s)
櫻井健郎, 芹澤滋子, 小林 淳, 児玉圭太, 李 政勲, 牧 秀明, 頭士泰之, 今泉圭隆, 鈴木規之, 堀口敏宏, 白石寛明
Organizer
第24回環境化学討論会
Place of Presentation
札幌コンベンションセンター（北海道札幌市）
Year and Date
2015-06-25
Related Report
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[Presentation] PFOSおよびPFOAの東京湾への流入速度の経年変化(2004-2010)2015
Author(s)
櫻井健郎, 芹澤滋子, 児玉圭太, 堀口敏宏, 牧 秀明, 頭士泰之, 今泉圭隆, 鈴木規之, 白石寛明, 小林 淳, 李 政勲
Organizer
第49回日本水環境学会年会
Place of Presentation
金沢大学, 石川県金沢市
Year and Date
2015-03-17
Related Report
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[Presentation] Emission of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) in the Tokyo-Bay catchment basin, Japan in 2004-20102014
Author(s)
Sakurai T, Serizawa S, Kobayashi J, Kodama K, Lee J, Maki H, Imaizumi Y, Suzuki N, Horiguchi T, Shiraishi H
Organizer
Society of Environmental Toxicology and Chemistry North America 35th Annual Meeting
Place of Presentation
Vancouver Convention Center, Vancouver, B.C., Canada
Year and Date
2014-11-12
Related Report
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[Presentation] 東京湾に流入する淡水中のPFOS およびPFOA濃度の経年変化（2004–2010)2014
Author(s)
櫻井健郎, 芹澤滋子, 小林 淳, 児玉圭太, 李 政勲, 牧 秀明, 今泉圭隆, 鈴木規之, 堀口敏宏, 白石寛明
Organizer
第23回環境化学討論会
Place of Presentation
京都大学百周年記念時計台記念館, 京都府京都市
Year and Date
2014-05-14
Related Report
